04-448 | Anti-HSP27 Phospho (Ser82) Antibody, rabbit monoclonal

100 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RIF, IP, WB, IH(P)RbPurifiedMonoclonal Antibody
      Catalogue Number04-448
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-HSP27 Phospho (Ser82) Antibody, rabbit monoclonal
      Product Information
      PresentationIn 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-HSP27 Phospho (Ser82) Antibody, rabbit detects level of HSP27 Phospho (Ser82) & has been published & validated for use in IF, IP, WB, IH(P).
      Key Applications
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Biological Information
      ImmunogenKLH-conjugated synthetic phosphor-peptide composed of residues juxtaposed to Serine 82 of human HSP27.
      SpecificityRecognizes a region composed of residues juxtaposed to Serine 82 of human HSP27.
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HSPB1
      • Hs.76067
      • HSP27
      • Hsp25
      • HspB1
      • HSP28
      • CMT2F
      • HS.76067
      • DKFZp586P1322
      • HMN2B
      • SRP27
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04792 # Involved in stress resistance and actin organization.
      SIZE: 205 amino acids; 22783 Da SUBUNIT: Interacts with TGFB1I1 (By similarity). Associates with alpha- and beta-tubulin and microtubules. Interacts with HSPB8 and HSPBAP1.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock.
      PTM: Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
      DISEASE: SwissProt: P04792 # Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot- Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant. & Defects in HSPB1 are a cause of distal hereditary motor neuropathy (dHMN) [MIM:608634]. Distal HMN is a pure motor peripheral neuropathy without sensory abnormalities.
      SIMILARITY: SwissProt: P04792 ## Belongs to the small heat shock protein (HSP20) family.
      Molecular Weight27 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      Anti-HSP27 Phospho (Ser82) - DAM1423797 DAM1423797
      Anti-HSP27 Phospho (Ser82) - DAM1588277 DAM1588277
      Anti-HSP27 Phospho (Ser82) - DAM1718116 DAM1718116
      Anti-HSP27 Phospho (Ser82) - JBC1510631 JBC1510631
      Anti-HSP27 Phospho (Ser82), Rabbit monoclonal - JBC1371304 JBC1371304


      Reference overviewApplicationSpeciesPub Med ID
      Distinct roles of MK2 and MK5 in cAMP/PKA- and stress/p38MAPK-induced heat shock protein 27 phosphorylation.
      Shiryaev, A; Dumitriu, G; Moens, U
      Journal of molecular signaling  6  4  2011

      Show Abstract Full Text Article
      21575178 21575178
      PRKC-ζ Expression Promotes the Aggressive Phenotype of Human Prostate Cancer Cells and Is a Novel Target for Therapeutic Intervention.
      Yao, S; Bee, A; Brewer, D; Dodson, A; Beesley, C; Ke, Y; Ambroisine, L; Fisher, G; Møller, H; Dickinson, T; Gerard, P; Lian, LY; Risk, J; Lane, B; Smith, P; Reuter, V; Berney, D; Gosden, C; Scardino, P; Cuzick, J; Djamgoz, MB; Cooper, C; Foster, CS
      Genes & cancer  1  444-64  2010

      Show Abstract
      Western BlottingHuman21779455 21779455
      The 28-kDa protein whose phosphorylation is induced by protein kinase C activators in MCF-7 cells belongs to the family of low molecular mass heat shock proteins and is the estrogen-regulated 24-kDa protein
      Faucher, C, et al
      J Biol Chem, 268:15168-73 (1993)  1993

      8325890 8325890
      The 29-kDa proteins phosphorylated in thrombin-activated human platelets are forms of the estrogen receptor-related 27-kDa heat shock protein
      Mendelsohn, M E, et al
      Proc Natl Acad Sci USA, 88:11212-6 (1991)  1991

      1763035 1763035

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      Life Science Research > Antibodies and Assays > Primary Antibodies