05-775 | Anti-Ha-Ras Antibody, clone MC57, rabbit monoclonal

05-775
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-775
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Ha-Ras Antibody, clone MC57, rabbit monoclonal
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationThis Anti-Ha-Ras Antibody, clone MC57 is validated for use in WB for the detection of Ha-Ras.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST fusion protein corresponding to full length human Ha-Ras
      CloneMC57
      HostRabbit
      SpecificityHa-Ras; does not cross react with Cdc42, Rho or Rac1
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NotePredicted to cross-react with mouse and rat based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HRAS
      • Ha-Ras
      • K-ras
      • N-ras
      • p21ras
      • CTLO
      • HRAS1
      • c-bas/has
      • H-Ras-1
      • RASH1
      • c-H-ras
      • H-RasIDX
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01112 # Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
      SIZE: 189 amino acids; 21298 Da
      SUBUNIT: In its GTP-bound form interacts with PLCE1. Interacts with TBC1D10C. Interacts with RGL3 (By similarity).
      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Note=Shuttles between the plasma membrane and the Golgi apparatus.
      PTM: Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
      DISEASE: SwissProt: P01112 # Defects in HRAS are the cause of Costello syndrome [MIM:218040]; also known as faciocutaneoskeletal syndrome. Costello syndrome is a rare condition characterized by prenatally increased growth, postnatal growth deficiency, mental retardation, distinctive facial appearance, cardiovascular abnormalities (typically pulmonic stenosis, hypertrophic cardiomyopathy and/or atrial tachycardia), tumor predisposition, skin and musculoskeletal abnormalities. & Mutations which change positions 12, 13 or 61 activate the potential of c-ras to transform cultured cells and are implicated in a variety of human tumors. & Defects in HRAS are a cause of bladder cancer [MIM:109800]. & Defects in HRAS are also the cause of oral squamous cell carcinoma (OSCC).
      SIMILARITY: SwissProt: P01112 ## Belongs to the small GTPase superfamily. Ras family.
      Molecular Weight~21kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from A431 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Ha-Ras, clone MC57 - 26823 26823

      References

      Reference overviewPub Med ID
      Identification of novel Ras-cooperating oncogenes in Drosophila melanogaster: a RhoGEF/Rho-family/JNK pathway is a central driver of tumorigenesis.
      Brumby, AM; Goulding, KR; Schlosser, T; Loi, S; Galea, R; Khoo, P; Bolden, JE; Aigaki, T; Humbert, PO; Richardson, HE
      Genetics  188  105-25  2011

      Show Abstract
      21368274 21368274
      Loss of human Scribble cooperates with H-Ras to promote cell invasion through deregulation of MAPK signalling.
      Dow, LE; Elsum, IA; King, CL; Kinross, KM; Richardson, HE; Humbert, PO
      Oncogene  27  5988-6001  2008

      Show Abstract
      18641685 18641685
      Mammalian Ras interacts directly with the serine/threonine kinase Raf.
      Vojtek, A B, et al.
      Cell, 74: 205-14 (1993)  1993

      Show Abstract
      8334704 8334704

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies