07-955 | Anti-Hexim 1 Antibody

07-955
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, ChpWB, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-955
      DescriptionAnti-Hexim 1 Antibody
      Alternate Names
      • Cardiac lineage protein 1
      • Estrogen down-regulated gene 1 protein
      • Hexamethylene bis-acetamide-inducible protein 1
      • Menage a quatre protein 1
      • hexamethylene bis-acetamide inducible 1
      • hexamethylene bisacetamide-inducible protein
      • hexamethylene-bis-acetamide-inducible transcript 1
      • hexamthylene bis-acetamide inducible 1
      • menage a quatre 1
      Background InformationHexim1 is a transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. Hexim1 works in cooperation with 7SK snRNA to sequester P-TEFb in a large inactive 7SK snRNP complex, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK snRNP complex is composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), Hexim1, Hexim2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Hexim1 also interacts with the N-CoR complex through NCOR1, ESR1, NR3C1, and may interact with NF-kappa-B through REL A. Hexim1 is located in the nucleus where it can bind with alpha-importin, and can also be found in the cytoplasm. Hexim1 is up-regulated by HMBA (hexamethylene bisacetamide) at the protein level and down-regulated by estrogen.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationAnti-Hexim 1 Antibody is a Rabbit Polyclonal Antibody for detection of Hexim 1 also known as Hexamethylene bis-acetamide-inducible protein 1 & has been validated in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesWestern Blot (SNAP ID) Analysis: 4 µg/ml from a previous lot detected Hexim 1 on 10 µg of HeLa cell lysate.

      Immunoprecipitation Analysis: A representative lot of this antibody was used by an independent laboratory for IP.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Hexim 1 at and around the N-terminus.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Hexim1 at the N-terminus.
      Species Reactivity
      • Human
      • Chimpanzee
      Species Reactivity NoteDemonstrated to react with human. Predicted to react with chimpanzee and Rhesus monkey based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryExpression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns.
      Gene Symbol
      • CLP-1
      • CLP1
      • EDG1
      • HIS1
      • HMBA-inducible
      • MAQ1
      • Hexim1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity.

      SUBUNIT STRUCTURE: Homo-oligomer and hetero-oligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA.

      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Binds alpha-importin and is mostly nuclear.

      TISSUE SPECIFICITY: Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues.

      INDUCTION: Up-regulated by HMBA (hexamethylene bisacetamide) (at protein level). Down-regulated by estrogen.

      DOMAIN: The coiled-coil domain mediates oligomerization.

      MISCELLANEOUS: Inhibits Tat activity which is required for HIV-1 transcription.

      SEQUENCE SIMILARITIES: Belongs to the HEXIM family.
      Molecular Weight53 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.1 µg/ml of this antibody detected Hexim 1 on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies