06-1291 | Anti-Hexim1 Antibody

06-1291
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBShSerumPolyclonal Antibody
      Description
      Catalogue Number06-1291
      DescriptionAnti-Hexim1 Antibody
      Alternate Names
      • Cardiac lineage protein 1
      • Estrogen down-regulated gene 1 protein
      • Hexamethylene bis-acetamide-inducible protein 1
      • Menage a quatre protein 1
      • hexamethylene bis-acetamide inducible 1
      • hexamethylene bisacetamide-inducible protein
      • hexamethylene-bis-acetamide-inducible transcript 1
      • hexamthylene bis-acetamide inducible 1
      • menage a quatre 1
      Background InformationHexim1 is a transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. Hexim1 works in cooperation with 7SK snRNA to sequester P-TEFb in a large inactive 7SK snRNP complex, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. Hexim1 may also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. The 7SK snRNP complex is composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), Hexim1, Hexim2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Hexim1 also interacts with the N-CoR complex through NCOR1, ESR1, NR3C1, and may interact with NF-kappa-B through REL A. Hexim1 is located in the nucleus where it can bind with alpha-importin, and can also be found in the cytoplasm. Hexim1 is up-regulated by HMBA (hexamethylene bisacetamide) at the protein level and down-regulated by estrogen.
      References
      Product Information
      FormatSerum
      Control
      • HeLa cell lysate
      PresentationSheep polyclonal serum containing 0.05% sodium azide.
      Applications
      ApplicationAnti-Hexim1 Antibody is a Sheep polyclonal antibody for detection of Hexim1 also known as Cardiac lineage protein 1, Estrogen down-regulated gene 1 protein ,Hexamethylene bis-acetamide-inducible protein 1 & has been validated in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis: A 1:1,000 dilution from a previous lot detected Hexim1 in 10 µg of HeLa cell lysate.
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to human Hexim1.
      HostSheep
      SpecificityThis antibody recognizes Hexim1.
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react with human.
      Mouse (85% sequence homology) and rat (86% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryExpression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns.
      Gene Symbol
      • CLP-1
      • CLP1
      • EDG1
      • FLJ13562
      • HIS1
      • HMBA-inducible
      • MAQ1
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity.
      SUBUNIT STRUCTURE: Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA.
      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Binds alpha-importin and is mostly nuclear.
      TISSUE SPECIFICITY: Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues.
      INDUCTION: Up-regulated by HMBA (hexamethylene bisacetamide) (at protein level). Down-regulated by estrogen.
      DOMAIN: The coiled-coil domain mediates oligomerization.
      MISCELLANEOUS: Inhibits Tat activity which is required for HIV-1 transcription.
      SEQUENCE SIMILARITIES: Belongs to the HEXIM family.
      Molecular Weight~40 kDa (predicted).
      Molecular weight Note: According to an independent laboratory, the two isoforms of Hexim1 run at ~53 kDa & ~68 kDa. This antibody can detect the ~53 kDa isoform in multiple cells.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.
      Western Blot Analysis: A 1:1,000 dilution of this antibody detected Hexim1 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Hexim1 - NRG1637575 NRG1637575

      References

      Reference overviewApplicationPub Med ID
      The mechanism of release of P-TEFb and HEXIM1 from the 7SK snRNP by viral and cellular activators includes a conformational change in 7SK.
      Krueger, BJ; Varzavand, K; Cooper, JJ; Price, DH
      PloS one  5  e12335  2010

      Show Abstract
      Western Blotting20808803 20808803

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies