04-1664 | Anti-Histidine Tag Antibody, clone RM146

04-1664
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      A Broad Range Of SpeciesWB, IPRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1664
      DescriptionAnti-Histidine Tag Antibody, clone RM146
      References
      Product Information
      FormatPurified
      PresentationRabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
      Applications
      ApplicationThis Anti-Histidine Tag Antibody, clone RM146 is validated for use in Western Blotting, Immunoprecipitation for the detection of Histidine Tag.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis (IP): 0.5-2 μg/mL from a representative lot detected Histidine Tag in HEK293T whole lysate control and IP by clone RM146.
      Biological Information
      ImmunogenMixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
      CloneRM146
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
      IsotypeIgG
      Species Reactivity
      • A Broad Range Of Species
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein A Purfied
      Molecular Weight~52 and ~85 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293T cell lysate untransfected with His-Tag fusion protein X, transfected with His-Tag fusion protein X, E. coli lysate without His-Tag Protein Y, and E. coli lysate with His-Tag Protein Y.

      Western Blotting Analysis (WB): 0.2 μg/mL of this antibody detected Histidine Tag in HEK293T cell lysate transfected with His-Tag fusion protein X and E. coli lysate with His-Tag Protein Y.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications