05-531 | Anti-Histidine Tagged Antibody, clone 4D11

05-531
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-531
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Histidine Tagged Antibody, clone 4D11
      References
      Product Information
      FormatPurified
      Control
      • Cells expressing the His-tag
      PresentationProtein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.014M phosphate buffer, pH 7.6, 0.175M NaCl, and 0.07% sodium azide.
      Applications
      ApplicationThis Anti-Histidine Tagged Antibody (anti His antibody) clone 4D11 is validated for use in IC, WB for the detection of Histidine Tagged.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Biological Information
      ImmunogenN-terminal His-tagged and truncated TRAP protein corresponding to amino acids 26-248 from P. falciparum expressed in E coli.
      Clone4D11
      HostMouse
      SpecificityRecognizes tag sequence HHHHHHGS on either the N- or C-terminus of recombinant proteins; GS amino acids are required for recognition of 6His tag
      IsotypeIgG2aκ
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      Molecular WeightThe apparent molecular weight is related to the tagged protein.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on recombinant N-terminal and C-terminal His-tagged proteins
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-His Tag, clone 4D11 2465220
      Anti-His Tag, clone 4D11 (mouse monoclonal IgG2a#954;) - 2138012 2138012
      Anti-His Tag, clone 4D11 (mouse monoclonal IgG2aκ) - 2383523 2383523
      Anti-His Tag, clone 4D11 (mouse monoclonal IgG2aK) 2927603
      Anti-His Tag, clone 4D11 (mouse monoclonal IgG2ak) - 2032282 2032282
      Anti-His Tag, clone 4D11 (mouse monoclonal IgG2ak) - JBC1940045 JBC1940045
      Anti-His Tag, clone 4D11 - 19490 19490
      Anti-His Tag, clone 4D11 - 1972316 1972316
      Anti-His Tag, clone 4D11 - 23334 23334
      Anti-His Tag, clone 4D11 - 28132 28132

      References

      Reference overviewApplicationSpeciesPub Med ID
      Na+ channel-dependent recruitment of Navβ4 to axon initial segments and nodes of Ranvier.
      Buffington, SA; Rasband, MN
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  6191-202  2013

      Show Abstract
      Rat23554500 23554500
      PRAME is a golgi-targeted protein that associates with the Elongin BC complex and is upregulated by interferon-gamma and bacterial PAMPs.
      Wadelin, FR; Fulton, J; Collins, HM; Tertipis, N; Bottley, A; Spriggs, KA; Falcone, FH; Heery, DM
      PloS one  8  e58052  2013

      Show Abstract
      Western Blotting23460923 23460923
      Thrombospondin-related adhesive protein (TRAP) of Plasmodium falciparum: expression during sporozoite ontogeny and binding to human hepatocytes.
      Robson, K J, et al.
      EMBO J., 14: 3883-94 (1995)  1995

      Show Abstract
      7664729 7664729

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies