05-457 | Anti-Histone H1 Antibody, clone AE-4

05-457
200 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, B, PlELISA, ICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-457
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Histone H1 Antibody, clone AE-4
      Alternate Names
      • H1 histone family, member 4
      • Histone H1b
      • histone 1
      • H1e
      • histone cluster 1
      • H1e
      Background InformationHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
      References
      Product Information
      FormatPurified
      Control
      • Nuclear extracts from NIH3T3 or HeLa cells.
      PresentationPurified mouse monoclonal IgG2a in buffer containing PBS, pH 7.2, with 0.1% sodium azide. Frozen solution.
      Applications
      ApplicationUse Anti-Histone H1 Antibody, clone AE-4 (mouse monoclonal antibody) validated in ELISA, ICC, WB to detect Histone H1 also known as H1 histone family member 4, Histone H1b, histone 1 H1e, histone cluster 1 H1e.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry:
      5-20 µg/mL of a previous lot showed positive nuclear immunostaining in A431 cells fixed with 4% paraformaldehyde and methanol.

      ELISA:
      Recommended.
      Biological Information
      ImmunogenNuclei from myeloid leukemia biopsy cells. Clone AE-4.
      CloneAE-4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes histone H1, Mr 32-33 kDa. Doublet detected due to hyperphosphorylation of histone H1 subpopulation. Cross-reacts with other histone isoforms in the following order of signal strength: H3, H2B > H4 > H2A, as determined with recombinant protein.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Bovine
      • Green Plants
      Species Reactivity NoteHuman and bovine. Predicted to stain nuclei from other animal and plant species.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • HIST1H1E
      • H1F4
      • H1.4
      • dJ221C16.5
      • MGC116819
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P10412 # Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
      SIZE: 219 amino acids; 21865 Da
      SUBCELLULAR LOCATION: Nucleus.
      SIMILARITY: SwissProt: P10412 ## Belongs to the histone H1/H5 family.
      MISCELLANEOUS: This variant accounts for 60% of histone H1.
      Molecular Weight32-33 kDa (Histone H1); Doublet (hyperphosphorylated Histone H1); 17 kDa (other histone isoforms).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on lysates from an acid extract of HeLa cells.

      Western Blot:
      1-4 µg/mL of this lot detected histone H1 in lysates from an acid extract of HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Histone H1, clone AE-4 - 2113348 2113348
      Anti-Histone H1, clone AE-4 - 2155592 2155592
      Anti-Histone H1, clone AE-4 - 2383461 2383461
      Anti-Histone H1, clone AE-4 - 2387415 2387415
      Anti-Histone H1, clone AE-4 - 2420363 2420363
      Anti-Histone H1, clone AE-4 - 2428463 2428463
      Anti-Histone H1, clone AE-4 - DAM1734751 DAM1734751
      Anti-Histone H1, clone AE-4 - 16261 16261
      Anti-Histone H1, clone AE-4 - 17735 17735
      Anti-Histone H1, clone AE-4 - 2019721 2019721

      References

      Reference overviewApplicationPub Med ID
      Super-resolution microscopy reveals decondensed chromatin structure at transcription sites.
      Wang, Y; Maharana, S; Wang, MD; Shivashankar, GV
      Scientific reports  4  4477  2014

      Show Abstract
      24667378 24667378
      Deimination of linker histones links neutrophil extracellular trap release with autoantibodies in systemic autoimmunity.
      Dwivedi, N; Neeli, I; Schall, N; Wan, H; Desiderio, DM; Csernok, E; Thompson, PR; Dali, H; Briand, JP; Muller, S; Radic, M
      FASEB journal : official publication of the Federation of American Societies for Experimental Biology  28  2840-51  2014

      Show Abstract
      24671707 24671707
      Nicotine induces chromatin remodelling through decreases in the methyltransferases GLP, G9a, Setdb1 and levels of H3K9me2.
      Chase, KA; Sharma, RP
      The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP)  16  1129-38  2013

      Show Abstract
      23067581 23067581
      Novel alterations in the epigenetic signature of MeCP2-targeted promoters in lymphocytes of Rett syndrome patients.
      Lilja, T; Wallenborg, K; Björkman, K; Albåge, M; Eriksson, M; Lagercrantz, H; Rohdin, M; Hermanson, O
      Epigenetics  8  246-51  2013

      Show Abstract
      23348913 23348913
      High-resolution mapping of h1 linker histone variants in embryonic stem cells.
      Cao, K; Lailler, N; Zhang, Y; Kumar, A; Uppal, K; Liu, Z; Lee, EK; Wu, H; Medrzycki, M; Pan, C; Ho, PY; Cooper, GP; Dong, X; Bock, C; Bouhassira, EE; Fan, Y
      PLoS genetics  9  e1003417  2013

      Show Abstract
      23633960 23633960
      Prostate cancer stem cell-targeted efficacy of a new-generation taxoid, SBT-1214 and novel polyenolic zinc-binding curcuminoid, CMC2.24.
      Botchkina, GI; Zuniga, ES; Rowehl, RH; Park, R; Bhalla, R; Bialkowska, AB; Johnson, F; Golub, LM; Zhang, Y; Ojima, I; Shroyer, KR
      PloS one  8  e69884  2013

      Show Abstract
      24086245 24086245
      Chromatin context and ncRNA highlight targets of MeCP2 in brain.
      Maxwell, SS; Pelka, GJ; Tam, PP; El-Osta, A
      RNA biology  10  1741-57  2013

      Show Abstract
      24270455 24270455
      Vitamin D receptor activators upregulate and rescue podocalyxin expression in high glucose-treated human podocytes.
      Verouti, SN; Tsilibary, EC; Fragopoulou, E; Iatrou, C; Demopoulos, CA; Charonis, AS; Charonis, SA; Drossopoulou, GI
      Nephron. Experimental nephrology  122  36-50  2012

      Show Abstract
      Fluorescence Activated Cell Sorting (FACS)23548800 23548800
      Histone h1 depletion impairs embryonic stem cell differentiation.
      Zhang, Y; Cooke, M; Panjwani, S; Cao, K; Krauth, B; Ho, PY; Medrzycki, M; Berhe, DT; Pan, C; McDevitt, TC; Fan, Y
      PLoS genetics  8  e1002691  2012

      Show Abstract
      22589736 22589736
      Sublethal doses of β-amyloid peptide abrogate DNA-dependent protein kinase activity.
      Cardinale, A; Racaniello, M; Saladini, S; De Chiara, G; Mollinari, C; de Stefano, MC; Pocchiari, M; Garaci, E; Merlo, D
      The Journal of biological chemistry  287  2618-31  2012

      Show Abstract
      22139836 22139836

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      06-1358 Anti-acetyl (Lys34) phospho (Ser36) Histone H1 Antibody Show Pricing & Availability
      06-1359 Anti-acetyl-Histone H1 (Lys46) Antibody Show Pricing & Availability
      06-1372 Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody Show Pricing & Availability
      MAB1276 Anti-Nuclei & Chromosomes Antibody, histone H1 protein, clone 1415-1 Show Pricing & Availability
      MAB3864 Anti-DNA/Histone H1 Antibody Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies