09-862 | Anti-Histone H2A.Z Antibody

09-862
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, H, Zebrafish, Rb, R, Xn, MWBShAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number09-862
      DescriptionAnti-Histone H2A.Z Antibody
      Alternate Names
      • H2A histone family, member Z
      • H2AZ histone
      Background InformationH2A.Z, also known as H2AFZ, is a variant of histone H2A variant that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa cell lysate.
      PresentationPurified sheep polyclonal IgG in PBS, pH 7.4 containing with 1% BSA with 0.02% sodium azide.
      Applications
      ApplicationUse Anti-Histone H2A.Z Antibody (Sheep Polyclonal Antibody) validated in WB to detect Histone H2A.Z also known as H2A histone family member Z, H2AZ histone.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenThe immunogen was a linear peptide corresponding to C-terminal amino acids 113-127 of human Histone H2A.Z.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostSheep
      SpecificityThis antibody recognizes Histone H2A.Z.
      Species Reactivity
      • Chicken
      • Human
      • Zebrafish
      • Rabbit
      • Rat
      • Xenopus
      • Mouse
      Species Reactivity NoteHuman. Predicted to cross-react with zebrafish, mouse, chicken, rat, rabbit, and Xenopus based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. Several transcript variants encoding different isoforms, have been identified for this gene.
      Gene Symbol
      • H2A.z
      • H2A/z
      • H2AZ
      • MGC117173
      • H2AFZ
      UniProt Number
      UniProt SummaryFUNCTION: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. Ref.7

      SUBUNIT STRUCTURE:The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms an heterodimer with H2B. H2AFZ interacts with INCENP By similarity.

      SUBCELLULAR LOCATION:Nucleus.

      PTM: Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.

      Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis By similarity.

      Not phosphorylated By similarity.

      SIMILARITY: Belongs to the histone H2A family.

      MOLECULAR WEIGHT: is 13413.4 Da from positions 2 - 128. Determined by ESI. Monoisotopic, not modified. Ref.8 Ref.10 Ref.11

      Molecular Weight~15 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis:
      1:1,000 dilution of this lot detected Histone H2A.Z in 10 μg of HeLa cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 years at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Histone H2A.Z - 2459505 2459505
      Anti-Histone H2A.Z - 2326439 2326439
      Anti-Histone H2A.Z - NG1590601 NG1590601
      Anti-Histone H2A.Z - NG1940773 NG1940773
      Anti-Histone H2A.Z -2726198 2726198
      Anti-Histone H2A.Z -2746235 2746235
      Anti-Histone H2A.Z Polyclonal Antibody 2887687
      Anti-Histone H2A.Z Polyclonal Antibody 2949950

      References

      Reference overviewPub Med ID
      The histone variant composition of centromeres is controlled by the pericentric heterochromatin state during the cell cycle.
      Boyarchuk, E; Filipescu, D; Vassias, I; Cantaloube, S; Almouzni, G
      Journal of cell science  127  3347-59  2014

      Show Abstract
      24906798 24906798
      Pancreatic islet enhancer clusters enriched in type 2 diabetes risk-associated variants.
      Pasquali, L; Gaulton, KJ; Rodríguez-Seguí, SA; Mularoni, L; Miguel-Escalada, I; Akerman, I; Tena, JJ; Morán, I; Gómez-Marín, C; van de Bunt, M; Ponsa-Cobas, J; Castro, N; Nammo, T; Cebola, I; García-Hurtado, J; Maestro, MA; Pattou, F; Piemonti, L; Berney, T; Gloyn, AL; Ravassard, P; Gómez-Skarmeta, JL; Müller, F; McCarthy, MI; Ferrer, J
      Nature genetics  46  136-43  2014

      Show Abstract
      24413736 24413736
      Histone H2A.Z and DNA methylation are mutually antagonistic chromatin marks.
      Zilberman, Daniel, et al.
      Nature, 456: 125-9 (2008)  2008

      Show Abstract
      18815594 18815594
      A new fluorescence resonance energy transfer approach demonstrates that the histone variant H2AZ stabilizes the histone octamer within the nucleosome.
      Park, Young-Jun, et al.
      J. Biol. Chem., 279: 24274-82 (2004)  2004

      Show Abstract
      15020582 15020582
      Histone H2A variants H2AX and H2AZ.
      Redon, Christophe, et al.
      Curr. Opin. Genet. Dev., 12: 162-9 (2002)  2002

      Show Abstract
      11893489 11893489

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies