05-1352 | Anti-Histone H2B Antibody, clone 5HH2-2A8

05-1352
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, RWB, ICCMAscitesMonoclonal Antibody
      Description
      Catalogue Number05-1352
      ReplacesMABE15
      DescriptionAnti-Histone H2B Antibody, clone 5HH2-2A8
      Alternate Names
      • H2B
      • Histone H2B
      • histone 2, H2be
      • histone cluster 2, H2be
      • H2B histone family, member Q
      Background InformationHistones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the 'beads on a string' structure.
      References
      Product Information
      FormatAscites
      Control
      • HepG2 cell lysate, L6 cell lysate, Mouse Brain Lysate
      PresentationMouse monoclonal IgG1κ ascites with 0.05% sodium azide.
      Applications
      ApplicationAnti-Histone H2B Antibody, clone 5HH2-2A8 is a Mouse Monoclonal Antibody for detection of Histone H2B also known as Histone H2B, histone 2 H2be & has been validated in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry:
      A 1:500 dilution of a representative lot of this antibody detected Histone H2B in A431, HeLa and NIH/3T3 cells.
      Biological Information
      ImmunogenOvalbumin-conjugated synthetic linear peptide corresponding to the C-terminus of Histone H2B.
      EpitopeC-terminus
      Cloneclone 5HH2-2A8
      HostMouse
      SpecificityAntibody recognizes Histone H2B near the C-terminus.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]
      Gene Symbol
      • H2B
      • GL105
      • H2B-GL105
      • H2B.1
      • H2B.q
      • H2B/q
      • H2BFQ
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q8N257 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

      SIZE: 126 amino acids; 13908 Da

      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

      SUBCELLULAR LOCATION: Nucleus.

      PTM: Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. & Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class- switch recombination.

      SIMILARITY: SwissProt: Q8N257 ## Belongs to the histone H2B family.
      Molecular Weight~15 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot in HepG2 cell lysate.

      Western Blot Analysis:
      A 1:1,000-1:2,000 dilution of this antibody detected Histone H2B in 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Histone H2B, clone 5HH2-2A8 - 2433429 2433429
      Anti-Histone H2B, clone 5HH2-2A8 - 2009449 2009449
      Anti-Histone H2B, clone 5HH2-2A8 - 2068452 2068452
      Anti-Histone H2B, clone 5HH2-2A8 - 2227188 2227188
      Anti-Histone H2B, clone 5HH2-2A8 - 2495644 2495644
      Anti-Histone H2B, clone 5HH2-2A8 - 2561123 2561123
      Anti-Histone H2B, clone 5HH2-2A8 - NG1949595 NG1949595
      Anti-Histone H2B, clone 5HH2-2A8 - NRG1786656 NRG1786656
      Anti-Histone H2B, clone 5HH2-2A8 -2786035 2786035
      Anti-Histone H2B, clone 5HH2-2A8 -2808207 2808207

      References

      Reference overviewPub Med ID
      USP7 cooperates with SCML2 to regulate the activity of PRC1.
      Lecona, E; Narendra, V; Reinberg, D
      Molecular and cellular biology  35  1157-68  2015

      Show Abstract
      25605328 25605328