04-928 | Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal

04-928
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, H, M, R, Yeast (S. cerevisiae)WB, ChIPRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-928
      Replaces05-928
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal
      OverviewAn antibody that recognizes the C-terminus of histone H3 and is useful for identifying total H3 independant of post-translational modifications.
      Alternate Names
      • H3
      • Histone H3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatCulture Supernatant
      PresentationCultured supernantant in 0.05% sodium azide
      Applications
      ApplicationAnti-Histone H3 Antibody, CT, pan, clone A3S is a Rabbit Monoclonal Antibody for detection of Histone H3 also known as Histone H3 & has been validated in ChIP & WB.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blot Analysis: 1:500-1:2,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.

      Chromatin Immunoprecipitation:
      2 μL of of a previous lot of this antibody immunoprecipitated chromatin associated with Histone H3 from a wild type yeast lysate.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3
      CloneA3S
      HostRabbit
      SpecificityRecognizes Histone H3.
      IsotypeIgG
      Species Reactivity
      • Chicken
      • Human
      • Mouse
      • Rat
      • Yeast (S. cerevisiae)
      Species Reactivity NoteBroad species cross-reactivity expected due to sequence homology
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
      Gene Symbol
      • H3F3A
      • H3F3B
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in testicular cells.
      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot analysis.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Histone H3, CT, pan, clone A3S 2843172
      Anti-Histone H3, CT, pan, clone A3S - 2398904 2398904
      Anti-Histone H3, CT, pan, clone A3S - 2290990 2290990
      Anti-Histone H3, CT, pan, clone A3S -2510305 2510305
      Anti-Histone H3, CT, pan, clone A3S -2557626 2557626
      Anti-Histone H3, CT, pan, clone A3S -2621947 2621947
      Anti-Histone H3, CT, pan, clone A3S -2727195 2727195
      Anti-Histone H3, CT, pan, clone A3S -2806550 2806550
      Anti-Histone H3, CT, pan, clone A3S Monoclonal Antibody 2897200
      Anti-Histone H3, CT, pantropic, clone A3S - DAM1558096 DAM1558096

      References

      Reference overviewApplicationPub Med ID
      The Phaseolus vulgaris PvTRX1h gene regulates plant hormone biosynthesis in embryogenic callus from common bean.
      Barraza, A; Cabrera-Ponce, JL; Gamboa-Becerra, R; Luna-Martínez, F; Winkler, R; Álvarez-Venegas, R
      Frontiers in plant science  6  577  2015

      Show Abstract
      26284093 26284093
      Histone titration against the genome sets the DNA-to-cytoplasm threshold for the Xenopus midblastula transition.
      Amodeo, AA; Jukam, D; Straight, AF; Skotheim, JM
      Proceedings of the National Academy of Sciences of the United States of America  112  E1086-95  2015

      Show Abstract
      25713373 25713373
      Histone demethylase Jumonji D3 (JMJD3) as a tumor suppressor by regulating p53 protein nuclear stabilization.
      Ene, CI; Edwards, L; Riddick, G; Baysan, M; Woolard, K; Kotliarova, S; Lai, C; Belova, G; Cam, M; Walling, J; Zhou, M; Stevenson, H; Kim, HS; Killian, K; Veenstra, T; Bailey, R; Song, H; Zhang, W; Fine, HA
      PloS one  7  e51407  2012

      Show Abstract
      Immunohistochemistry23236496 23236496
      A novel domain in Set2 mediates RNA polymerase II interaction and couples histone H3 K36 methylation with transcript elongation.
      Kizer, Kelby O, et al.
      Mol. Cell. Biol., 25: 3305-16 (2005)  2005

      15798214 15798214
      Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast.
      Xiao, Tiaojiang, et al.
      Genes Dev., 17: 654-63 (2003)  2003

      Show Abstract
      12629047 12629047
      Histone H3 lysine 4 methylation is mediated by Set1 and required for cell growth and rDNA silencing in Saccharomyces cerevisiae.
      Briggs, S D, et al.
      Genes Dev., 15: 3286-95 (2001)  2001

      Show Abstract
      11751634 11751634

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      Life Science Research > Antibodies and Assays > Primary Antibodies