05-928 | Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal

05-928
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ch, R, M, Yeast (S. cerevisiae)WB, ChIPRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-928
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal
      OverviewAn antibody that recognizes the C-terminus of histone H3 and is useful for identifying total H3 independant of post-translational modifications.
      Alternate Names
      • H3
      • Histone H3
      • H3 histone, family 3A
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
      References
      Product Information
      FormatPurified
      Control
      • Acid extracted proteins from HeLa cells, HeLa nuclear extracts or acid extracted HeLa cells treated with sodium butyrate.
      PresentationPurified rabbit monoclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.
      Applications
      ApplicationUse Anti-Histone H3 Antibody, CT, pan, clone A3S (Rabbit Monoclonal Antibody) validated in ChIP, WB to detect Histone H3 also known as Histone H3.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      2 μL of this antibody immunoprecipitated chromatin associated with Histone H3 from a wild type yeast lysate.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3.
      EpitopeC-terminus
      CloneA3S
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Histone H3, Mr 17 kDa
      IsotypeIgG
      Species Reactivity
      • Human
      • Chicken
      • Rat
      • Mouse
      • Yeast (S. cerevisiae)
      Species Reactivity NoteHuman, mouse, rat, yeast and chicken. Broad species cross-reactivity expected due to sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures.
      Gene Symbol
      • H3F3A
      • MGC87783
      • H3.3A
      • MGC87782
      • H3F3
      • H3.3B
      • H3F3B
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot analysis.

      Western Blot Analysis:
      1:2,000-1:20,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Histone H3, CT, pan, clone A3S - 0702052411 0702052411
      Anti-Histone H3, CT, pan, clone A3S - 2020536 2020536
      Anti-Histone H3, CT, pan, clone A3S - 2199770 2199770
      Anti-Histone H3, CT, pan, clone A3S - 2273166 2273166
      Anti-Histone H3, CT, pan, clone A3S - 2310361 2310361
      Anti-Histone H3, CT, pan, clone A3S - 2477921 2477921
      Anti-Histone H3, CT, pan, clone A3S - 30468 30468
      Anti-Histone H3, CT, pan, clone A3S - DAM1479571 DAM1479571
      Anti-Histone H3, CT, pan, clone A3S - DAM1658660 DAM1658660
      Anti-Histone H3, CT, pan, clone A3S - DAM1732849 DAM1732849

      References

      Reference overviewApplicationPub Med ID
      Synapsin IIb as a functional marker of submissive behavior.
      Nesher, E; Koman, I; Gross, M; Tikhonov, T; Bairachnaya, M; Salmon-Divon, M; Levin, Y; Gerlitz, G; Michaelevski, I; Yadid, G; Pinhasov, A
      Scientific reports  5  10287  2015

      Show Abstract
      25998951 25998951
      Identification of a new selective chemical inhibitor of mutant isocitrate dehydrogenase-1.
      Kim, HJ; Choi, BY; Keum, YS
      Journal of cancer prevention  20  78-83  2015

      Show Abstract
      25853107 25853107
      Steroid receptor coactivators 1 and 2 mediate fetal-to-maternal signaling that initiates parturition.
      Gao, L; Rabbitt, EH; Condon, JC; Renthal, NE; Johnston, JM; Mitsche, MA; Chambon, P; Xu, J; O'Malley, BW; Mendelson, CR
      The Journal of clinical investigation  125  2808-24  2015

      Show Abstract
      26098214 26098214
      Select 3',5'-cyclic nucleotide phosphodiesterases exhibit altered expression in the aged rodent brain.
      Kelly, Michy P, et al.
      Cell. Signal., 26: 383-97 (2014)  2014

      Show Abstract
      24184653 24184653
      Frequent and symmetric deposition of misfolded tau oligomers within presynaptic and postsynaptic terminals in Alzheimer's disease.
      Tai, HC; Wang, BY; Serrano-Pozo, A; Frosch, MP; Spires-Jones, TL; Hyman, BT
      Acta neuropathologica communications  2  146  2014

      Show Abstract
      25330988 25330988
      Global effects of the CSR-1 RNA interference pathway on the transcriptional landscape.
      Cecere, G; Hoersch, S; O'Keeffe, S; Sachidanandam, R; Grishok, A
      Nature structural & molecular biology  21  358-65  2014

      Show Abstract
      Western Blotting24681887 24681887
      Modularized functions of the Fanconi anemia core complex.
      Huang, Y; Leung, JW; Lowery, M; Matsushita, N; Wang, Y; Shen, X; Huong, D; Takata, M; Chen, J; Li, L
      Cell reports  7  1849-57  2014

      Show Abstract
      24910428 24910428
      A gnotobiotic mouse model demonstrates that dietary fiber protects against colorectal tumorigenesis in a microbiota- and butyrate-dependent manner.
      Donohoe, DR; Holley, D; Collins, LB; Montgomery, SA; Whitmore, AC; Hillhouse, A; Curry, KP; Renner, SW; Greenwalt, A; Ryan, EP; Godfrey, V; Heise, MT; Threadgill, DS; Han, A; Swenberg, JA; Threadgill, DW; Bultman, SJ
      Cancer discovery  4  1387-97  2014

      Show Abstract
      25266735 25266735
      ATM increases activation-induced cytidine deaminase activity at downstream S regions during class-switch recombination.
      Khair, L; Guikema, JE; Linehan, EK; Ucher, AJ; Leus, NG; Ogilvie, C; Lou, Z; Schrader, CE; Stavnezer, J
      Journal of immunology (Baltimore, Md. : 1950)  192  4887-96  2014

      Show Abstract
      24729610 24729610
      Vascular histone deacetylation by pharmacological HDAC inhibition.
      Rafehi, H; Balcerczyk, A; Lunke, S; Kaspi, A; Ziemann, M; Kn, H; Okabe, J; Khurana, I; Ooi, J; Khan, AW; Du, XJ; Chang, L; Haviv, I; Keating, ST; Karagiannis, TC; El-Osta, A
      Genome research  24  1271-84  2014

      Show Abstract
      Western Blotting24732587 24732587

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