05-499 | Anti-Histone H3 Antibody, clone 6.6.2

05-499
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Vrt, Pl, HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-499
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Histone H3 Antibody, clone 6.6.2
      OverviewThis monoclonal antibody was generated using an unmodified peptide corresponding to the first 20 amino acids of histone H3 and is specific for an unmodified epitope in that region.
      Alternate Names
      • H3 histone, family 3A
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell extract
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, with 0.05% sodium azide and 30% glycerol.
      Applications
      ApplicationAnti-Histone H3 Antibody, clone 6.6.2 is a Mouse Monoclonal Antibody for detection of Histone H3 also known as H3 histone family 3A & has been validated in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH conjugated peptide corresponding to amino acids 1-20 [ARTKQTARKSTGGKAPRKQLC] of human histone H3. Clone 6.6.2.
      Epitopea.a. 1-20
      Clone6.6.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes and is specific for histone H3, Mr 17kDa.
      IsotypeIgG1κ
      Species Reactivity
      • Vertebrates
      • Green Plants
      • Human
      Species Reactivity NoteHuman. This peptide sequence is identical in a wide range of animal and plant species.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1,with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • H3F3A
      • H3F3
      • MGC87782
      • H3.3B
      • MGC87783
      • H3F3B
      • H3.3A
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 136 amino acids; 15508 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).
      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on acid extracted lysates from HeLa cells.

      Western Blot Analysis:
      0.5-2 µg/mL of this lot detected Histone H3 in acid extracted lysates from HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Histone H3 (mouse monoclonal IgG1k) - DAM1437110 DAM1437110
      Anti-Histone H3 - 18006 18006
      Anti-Histone H3 - 21162 21162
      Anti-Histone H3 - 22511 22511
      Anti-Histone H3 - 23057 23057
      Anti-Histone H3 - 25198 25198
      Anti-Histone H3 - 31560 31560
      Anti-Histone H3, clone 6.6.2 2465188
      Anti-Histone H3, clone 6.6.2 - DAM1437110 DAM1437110
      Anti-Histone H3, clone 6.6.2 -2707891 2707891

      References

      Reference overviewApplicationSpeciesPub Med ID
      A methyltransferase required for proper timing of the vernalization response in Arabidopsis.
      Lee, J; Yun, JY; Zhao, W; Shen, WH; Amasino, RM
      Proceedings of the National Academy of Sciences of the United States of America  112  2269-74  2015

      Show Abstract
      25605879 25605879
      CSB-PGBD3 Mutations Cause Premature Ovarian Failure.
      Qin, Y; Guo, T; Li, G; Tang, TS; Zhao, S; Jiao, X; Gong, J; Gao, F; Guo, C; Simpson, JL; Chen, ZJ
      PLoS genetics  11  e1005419  2015

      Show Abstract
      26218421 26218421
      Enhanced trimethylation of histone h3 mediates impaired expression of hepatic glucose 6-phosphatase expression in offspring from rat dams exposed to hypoxia during pregnancy.
      Osumek, JE; Revesz, A; Morton, JS; Davidge, ST; Hardy, DB
      Reproductive sciences (Thousand Oaks, Calif.)  21  112-21  2014

      Show Abstract
      Western Blotting23744881 23744881
      Down-Regulation of miR-129-5p and the let-7 Family in Neuroendocrine Tumors and Metastases Leads to Up-Regulation of Their Targets Egr1, G3bp1, Hmga2 and Bach1.
      Døssing, KB; Binderup, T; Kaczkowski, B; Jacobsen, A; Rossing, M; Winther, O; Federspiel, B; Knigge, U; Kjær, A; Friis-Hansen, L
      Genes  6  1-21  2014

      Show Abstract
      25546138 25546138
      Antagonistic actions of Rcor proteins regulate LSD1 activity and cellular differentiation.
      Upadhyay, G; Chowdhury, AH; Vaidyanathan, B; Kim, D; Saleque, S
      Proceedings of the National Academy of Sciences of the United States of America  111  8071-6  2014

      Show Abstract
      Western Blotting24843136 24843136
      Identification of histone H3 clipping activity in human embryonic stem cells.
      Vossaert, L; Meert, P; Scheerlinck, E; Glibert, P; Van Roy, N; Heindryckx, B; De Sutter, P; Dhaenens, M; Deforce, D
      Stem cell research  13  123-34  2014

      Show Abstract
      Western BlottingHuman24874291 24874291
      Epigenetic regulation by chromatin activation mark H3K4me3 in primate progenitor cells within adult neurogenic niche.
      Sandstrom, RS; Foret, MR; Grow, DA; Haugen, E; Rhodes, CT; Cardona, AE; Phelix, CF; Wang, Y; Berger, MS; Lin, CH
      Scientific reports  4  5371  2014

      Show Abstract
      24947819 24947819
      Influence of Egr-1 in cardiac tissue-derived mesenchymal stem cells in response to glucose variations.
      Bastianelli, D; Siciliano, C; Puca, R; Coccia, A; Murdoch, C; Bordin, A; Mangino, G; Pompilio, G; Calogero, A; De Falco, E
      BioMed research international  2014  254793  2014

      Show Abstract
      Western Blotting24967343 24967343
      Molecular targets of chromatin repressive mark H3K9me3 in primate progenitor cells within adult neurogenic niches.
      Foret, MR; Sandstrom, RS; Rhodes, CT; Wang, Y; Berger, MS; Lin, CH
      Frontiers in genetics  5  252  2014

      Show Abstract
      Fluorescence Activated Cell Sorting (FACS)25126093 25126093
      Loss of Drosophila Ataxin-7, a SAGA subunit, reduces H2B ubiquitination and leads to neural and retinal degeneration.
      Mohan, RD; Dialynas, G; Weake, VM; Liu, J; Martin-Brown, S; Florens, L; Washburn, MP; Workman, JL; Abmayr, SM
      Genes & development  28  259-72  2014

      Show Abstract
      24493646 24493646

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