07-219 | Anti-Histone Macro H2A.1 Antibody

200 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICC, IHCRbAffinity PurifiedPolyclonal Antibody
      Catalogue Number07-219
      DescriptionAnti-Histone Macro H2A.1 Antibody
      Alternate Names
      • H2A histone family, member Y
      • histone macroH2A1.2
      • macroH2A1.2
      • Histone H2A.y
      • Histone macroH2A
      • histone macroH2A1.1
      • Medulloblastoma antigen MU-MB-50.205
      • core histone macro-H2A.1
      Background InformationHistone MacroH2A (mH2A) is a variant histone also known as H2AFY. mH2A is enriched in chromatin of the female inactive X chromosome and in highly methylated CpG loci. This histone variant is known to repress RNA polymerase II-driven transcription at the level of transcriptional initiation. In contrast to most histone variants containing only subtle changes, mH2A contains a large non-histone domain (the macro domain) on its C-terminus, resulting in a histone approximately three times the size of conventional H2A. There are 3 isoforms of mH2A, two of which (mH2A1.1, mH2A1.2 ) are formed by alternative splicing and another (mH2A2) from a separate gene. There are two phosphorylation sites within the basic hinge region of mH2A: Thr128 and Ser137. Phospho-Ser137 is enriched in mitosis, implicating mH2A in the regulation of posttranslational modifications throughout the cell cycle.
      Product Information
      FormatAffinity Purified
      • HeLa acid extract
      PresentationRabbit polyclonal antiserum IgG in buffer containing 0.05% sodium azide and 30% glycerol.
      ApplicationUse Anti-Histone Macro H2A.1 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, IHC to detect Histone Macro H2A.1 also known as H2A histone family member Y, histone macroH2A1.2.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunocytochemistry Analysis:
      1:500 dilution from a representative lot detected Histone macroH2A.1 in NIH/3T3, A431, and HeLa cells.

      Immunohistochemistry Analysis:
      A 1:50-1:200 dilution of a representative lot of this antibody was reported by an independent laboratory to stain hepatocyte nuclei from female mouse liver.
      Biological Information
      ImmunogenGST fusion-protein corresponding to the nonhistone region of rat Histone macroH2A1 (residues 160-370). .
      EpitopeMacro domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes Histone MacroH2A.1 at the macro domain.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • H2A.y
      • H2AF12M
      • H2AFJ
      • H2A/y
      • MACROH2A1.1
      • mH2A
      • MACROH2A
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.

      SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with HDAC1 and HDAC2 (By similarity).

      SUBCELLULAR LOCATION: Nucleus. Chromosome. Note: Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin. In quiescent lymphocytes, associated with centromeric constitutive heterochromatin.

      TISSUE SPECIFICTY: Ubiquitously expressed, with high levels in testis. Present in liver, kidney and adrenal gland (at protein level). In the liver, present in hepatocytes and at a lesser extent in cells of the bile ducts. In the kidney, present in proximal and distal convoluted tubules and in straight proximal tubules. In the adrenal gland, present in inner cells of the cortex and medulla.

      PTM: Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin (By similarity).

      SEQUENCE SIMILARITIES: Contains 1 histone H2A domain.

      Contains 1 Macro domain.
      Molecular Weight~ 40 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa acid extract.

      A 1:500-1:2000 dilution of this antibody detected Histone macroH2A1 in acid extracts of HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Histone macroH2A.1 - 2390438 2390438
      Anti-Histone macroH2A.1 - 2455677 2455677
      Anti-Histone macroH2A1 - 20465 20465
      Anti-Histone macroH2A1 - 24102 24102
      Anti-Histone macroH2A1 - 32188 32188
      Anti-Histone macroH2A1 - DAM1487556 DAM1487556
      Anti-Histone macroH2A1 - DAM1734772 DAM1734772
      Anti-Histone macroH2A1 - DAM1776452 DAM1776452
      Anti-Histone macroH2A1 - NG1830473 NG1830473


      Reference overviewApplicationSpeciesPub Med ID
      Xist Exon 7 Contributes to the Stable Localization of Xist RNA on the Inactive X-Chromosome.
      Yamada, N; Hasegawa, Y; Yue, M; Hamada, T; Nakagawa, S; Ogawa, Y
      PLoS genetics  11  e1005430  2015

      Show Abstract
      26244333 26244333
      Skp2-macroH2A1-CDK8 axis orchestrates G2/M transition and tumorigenesis.
      Xu, D; Li, CF; Zhang, X; Gong, Z; Chan, CH; Lee, SW; Jin, G; Rezaeian, AH; Han, F; Wang, J; Yang, WL; Feng, ZZ; Chen, W; Wu, CY; Wang, YJ; Chow, LP; Zhu, XF; Zeng, YX; Lin, HK
      Nature communications  6  6641  2015

      Show Abstract
      25818643 25818643
      MacroH2A1 downregulation enhances the stem-like properties of bladder cancer cells by transactivation of Lin28B.
      Park, SJ; Shim, JW; Park, HS; Eum, DY; Park, MT; Mi Yi, J; Choi, SH; Kim, SD; Son, TG; Lu, W; Kim, ND; Yang, K; Heo, K
      Oncogene  2015

      Show Abstract
      26028027 26028027
      Impact of flanking chromosomal sequences on localization and silencing by the human non-coding RNA XIST.
      Kelsey, AD; Yang, C; Leung, D; Minks, J; Dixon-McDougall, T; Baldry, SE; Bogutz, AB; Lefebvre, L; Brown, CJ
      Genome biology  16  208  2015

      Show Abstract
      26429547 26429547
      The histone variant MacroH2A1 regulates target gene expression in part by recruiting the transcriptional coregulator PELP1.
      Hussey, KM; Chen, H; Yang, C; Park, E; Hah, N; Erdjument-Bromage, H; Tempst, P; Gamble, MJ; Kraus, WL
      Molecular and cellular biology  34  2437-49  2014

      Show Abstract
      Chromatin Immunoprecipitation (ChIP)24752897 24752897
      Increased macroH2A1.1 expression correlates with poor survival of triple-negative breast cancer patients.
      Lavigne, AC; Castells, M; Mermet, J; Kocanova, S; Dalvai, M; Bystricky, K
      PloS one  9  e98930  2014

      Show Abstract
      Western Blotting24911873 24911873
      macroH2A1 histone variant represses rDNA transcription.
      Cong, R; Das, S; Douet, J; Wong, J; Buschbeck, M; Mongelard, F; Bouvet, P
      Nucleic acids research  42  181-92  2014

      Show Abstract
      24071584 24071584
      Dynamics of DOT1L localization and H3K79 methylation during meiotic prophase I in mouse spermatocytes.
      Ontoso, D; Kauppi, L; Keeney, S; San-Segundo, PA
      Chromosoma  123  147-64  2014

      Show Abstract
      Immunofluorescence24105599 24105599
      Histone H3.3 and its proteolytically processed form drive a cellular senescence programme.
      Duarte, LF; Young, AR; Wang, Z; Wu, HA; Panda, T; Kou, Y; Kapoor, A; Hasson, D; Mills, NR; Ma'ayan, A; Narita, M; Bernstein, E
      Nature communications  5  5210  2014

      Show Abstract
      25394905 25394905
      Macro histone variants are critical for the differentiation of human pluripotent cells.
      Barrero, MJ; Sese, B; Martí, M; Izpisua Belmonte, JC
      The Journal of biological chemistry  288  16110-6  2013

      Show Abstract
      Chromatin Immunoprecipitation (ChIP)Human23595991 23595991

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