06-494 | Anti-IκB-α Antibody

06-494
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-494
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-IκB-α Antibody
      Background InformationThe NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB-alpha protein. In response to many different NF-kappaB-inducing agentsincluding T-cell mitogens, proinflammatory cytokines, and viral transactivatorsthe inhibitory IkappaB-alpha is rapidly phosphorylated and degraded. Phosphorylation of IkappaB-alpha at Ser32 and Ser36 has been shown to stimulate conjugation with ubiquitin and subsequent degradation of IkappaB-alpha. Phosphorylation of IkappaB-alpha at Ser32 and Ser36 is critical for activation of NF-kappaB, which then translocates to the nucleus and induces transcription of genes that protect organism.
      References
      Product Information
      FormatPurified
      Control
      • Positive Control Included: Non-Stim A431 Cell Lysate (12-301). Other controls: NIH 3T3 cell lysate, HeLa cell lysate
      PresentationProtein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      Applications
      ApplicationDetect IκB-α with Anti-IκB-α Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenPurified GST-fusion protein containing full length human IκB-α/MAD-3 protein
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes IκB-α.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryNFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]
      Gene Symbol
      • NFKBIA
      • I-kappa-B-alpha
      • IKB-alpha
      • NFKBI
      • IKBA
      • MAD3
      • MAD-3
      • IkappaBalpha
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P25963 # Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. May be involved in regulation of transcriptional responses to NF-kappa-B, including cell adhesion, immune and proinflammatory responses, apoptosis, differentiation and growth. Controlled by sequential serine-phosphorylation, ubiquitination and degradation. Tyrosine-phosphorylation could only lead to dissociation from NF-kappa-B.
      SIZE: 317 amino acids; 35609 Da
      SUBUNIT: Interacts with p65 (RELA). Interacts with NKIRAS1 and NKIRAS2. Interacts with HBV protein X.
      SUBCELLULAR LOCATION: Cytoplasm.
      PTM: Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. & Ubiquitinated; subsequent to stimulus-dependent phosphorylation on serines, polyubiquitination targets the protein for rapid degradation via the ubiquitin system.
      DISEASE: SwissProt: P25963 # Defects in NFKBIA are a cause of autosomal dominant anhidrotic ectodermal dysplasia with immunodeficiency (AD-EDA-ID) [MIM:164008]. Ectodermal dysplasias (EDs) constitute a heterogeneous group of developmental disorders affecting tissues of ectodermal origin. EDs are characterized by abnormal development of two or more ectodermal structures such as hair, teeth, nails and sweat glands, with or without any additional clinical sign. Each combination of clinical features represents a different type of ectodermal dysplasia.
      SIMILARITY: SwissProt: P25963 ## Belongs to the NF-kappa-B inhibitor family. & Contains 5 ANK repeats.
      Molecular Weight38 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from A431 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-IkB-a - DAM1564519 DAM1564519
      Anti-Ikappa;B-alpha; - 13972 13972
      Anti-Ikappa;B-alpha; - 18042 18042
      Anti-Ikappa;B-alpha; - 22631 22631
      Anti-IκB-α (rabbit polyclonal IgG) 2470863

      References

      Reference overviewApplicationPub Med ID
      Chromatin-bound IκBα regulates a subset of polycomb target genes in differentiation and cancer.
      Mulero, MC; Ferres-Marco, D; Islam, A; Margalef, P; Pecoraro, M; Toll, A; Drechsel, N; Charneco, C; Davis, S; Bellora, N; Gallardo, F; López-Arribillaga, E; Asensio-Juan, E; Rodilla, V; González, J; Iglesias, M; Shih, V; Mar Albà, M; Di Croce, L; Hoffmann, A; Miyamoto, S; Villà-Freixa, J; López-Bigas, N; Keyes, WM; Domínguez, M; Bigas, A; Espinosa, L
      Cancer cell  24  151-66  2013

      Show Abstract
      23850221 23850221
      GCN5 is a required cofactor for a ubiquitin ligase that targets NF-kappaB/RelA.
      Xicheng Mao,Nathan Gluck,Duo Li,Gabriel N Maine,Haiying Li,Iram W Zaidi,Aparna Repaka,Marty W Mayo,Ezra Burstein
      Genes & development  23  2009

      Show Abstract Full Text Article
      19339690 19339690
      Synergistic activation of HIV-1 expression by deacetylase inhibitors and prostratin: implications for treatment of latent infection.
      Reuse, S; Calao, M; Kabeya, K; Guiguen, A; Gatot, JS; Quivy, V; Vanhulle, C; Lamine, A; Vaira, D; Demonte, D; Martinelli, V; Veithen, E; Cherrier, T; Avettand, V; Poutrel, S; Piette, J; de Launoit, Y; Moutschen, M; Burny, A; Rouzioux, C; De Wit, S; Herbein, G; Rohr, O; Collette, Y; Lambotte, O; Clumeck, N; Van Lint, C
      PloS one  4  e6093  2009

      Show Abstract
      Western Blotting19564922 19564922
      COMMD1 promotes the ubiquitination of NF-kappaB subunits through a cullin-containing ubiquitin ligase
      Maine, Gabriel N, et al
      EMBO J, 26:436-47 (2007)  2007

      17183367 17183367
      Involvement of nuclear factor-kappa B on corticosterone- induced rat Leydig cell apoptosis.
      Qian Wang, Hui-Bao Gao
      Asian journal of andrology  8  693-702  2006

      Show Abstract
      16855770 16855770
      Efficient nuclear export of p65-IkappaBalpha complexes requires 14-3-3 proteins.
      Aguilera, C; Fernández-Majada, V; Inglés-Esteve, J; Rodilla, V; Bigas, A; Espinosa, L
      Journal of cell science  119  3695-704  2006

      Show Abstract
      16931600 16931600
      COMMD proteins, a novel family of structural and functional homologs of MURR1.
      Burstein, E; Hoberg, JE; Wilkinson, AS; Rumble, JM; Csomos, RA; Komarck, CM; Maine, GN; Wilkinson, JC; Mayo, MW; Duckett, CS
      The Journal of biological chemistry  280  22222-32  2005

      Show Abstract
      Western Blotting15799966 15799966
      Recruitment of IkappaBalpha to the hes1 promoter is associated with transcriptional repression.
      Aguilera, C; Hoya-Arias, R; Haegeman, G; Espinosa, L; Bigas, A
      Proceedings of the National Academy of Sciences of the United States of America  101  16537-42  2004

      Show Abstract Full Text Article
      15536134 15536134
      Functional characterization of pkr gene products expressed in cells from mice with a targeted deletion of the N terminus or C terminus domain of PKR.
      Baltzis, D; Li, S; Koromilas, AE
      The Journal of biological chemistry  277  38364-72  2002

      Show Abstract
      12161430 12161430
      Differential activation of MAP kinase signaling pathways and nuclear factor-kappaB in bronchoalveolar cells of smokers and nonsmokers.
      K Mochida-Nishimura, K Surewicz, J V Cross, R Hejal, D Templeton, E A Rich, Z Toossi
      Molecular medicine (Cambridge, Mass.)  7  177-85  2001

      Show Abstract Full Text Article
      11471554 11471554

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      Included Positive Control

      Catalogue Number Description  
      12-301 Non-Stimulated A431 Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies