ABC939 | Anti-ICAT Antibody

ABC939
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IP, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC939
      DescriptionAnti-ICAT Antibody
      Alternate Names
      • Beta-catenin-interacting protein 1
      • ICAT
      • Inhibitor of beta-catenin and Tcf-4
      Background InformationBeta-catenin-interacting protein 1 (UniProt Q9NSA3; also known as Beta-catenin-interacting protein ICAT, Inhibitor of beta-catenin and Tcf-4) is encoded by the CTNNBIP1 (also known as ICAT) gene (Gene ID 56998) in human. Inhibitor of beta-catenin and TCF (ICAT) negatively regulates T-cell factor/lymphoid enhancer factor (TCF/LEF)–beta-catenin transcriptional activity by competing against TCF/LEF for beta-catenin binding. ICAT gene knockout leads to premature death of newborn mice with gut, kidney, and craniofacial anomalies, implicating an important role of ICAT in epithelial establishment/maintenance and neural crest cell differentiation. The motility of melanoma cells through 3D matrices in vitro relies on two interconvertible modes of movements. ICAT is reported to suppress the expression of developmentally downregulated 9 (NEDD9) of Wnt/beta-catenin signaling pathway in human Lu1205 melanoma cells, resulting in a shift from the Rac-dependent mesenchymal movement to the Rho/ROCK-dependent amoeboid motility in vitro and enhanced colonization of transplanted Lu1205 in the lungs of nude mice in vivo.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide.
      Applications
      ApplicationDetect ICAT/CTNNBIP1 using this rabbit Polyclonal Anti-ICAT Antibody, Cat. No. ABC939, validated for use in Immunocytochemistry, Western Blotting and Immunoprecipitation.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot co-immunoprecitated beta-catenin with ICAT from lysates of five human melanoma cell lines (Lu1205, WM852, Dauv-1, Mel501, and Skmel3) (Domingues, M.J., et al. (2014). Cancer Res. 74(7):1983-1995).
      Western Blotting Analysis: A representative lot detected ICAT in Lu1205, Mel501, human melanoma cells, and Skmel3 (Domingues, M.J., et al. (2014). Cancer Res. 74(7):1983-1995).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human ICAT.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Rat, Mouse, Bovine, Xenopus, Zebrafish, Chicken based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CTNNBIP1
      • ICAT
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~9 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431, HeLa, HUVEC and cells.

      Immunocytochemistry Analysis: 4 µg/mL of this antibody detected ICAT in A431, HeLa, HUVEC cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-ICAT -Q2581253 Q2581253

      References

      Reference overviewPub Med ID
      ?-catenin inhibitor ICAT modulates the invasive motility of melanoma cells.
      Domingues, MJ; Rambow, F; Job, B; Papon, L; Liu, W; Larue, L; Bonaventure, J
      Cancer research  74  1983-95  2014

      Show Abstract
      24514042 24514042

      Technical Info

      Title
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      White Paper: Further considerations of antibody validation and usage.