07-1008 | Anti-IKKβ Antibody

07-1008
150 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ca, Ch, B, Fe, MWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1008
      DescriptionAnti-IKKβ Antibody
      Alternate Names
      • I-kappa-B kinase 2
      • I-kappa-B-kinase beta
      • Nuclear factor NF-kappa-B inhibitor kinase beta 2
      Background InformationIKKβ (IkB Kinase, IKK beta, I-Kappa-B kinase-beta) is a member of the IKKcomplex which is composed of IKKalpha, IKKbeta, IKKgamma and IKAP. IkB Kinase (IKK) complex is a critical component in NFkB regulation. In a majority of unstimulated cells, the NFkB transcription factors exist in their inactive form and are retained in the cytoplasm by the bound inhibitory IkB proteins. For NFkB to become activated, it must first disassociate from the inhibitor IkB. This occurs via the serine phosphorylation of IkB on Ser32 and Ser36. Post receptor stimulation, most notably by TNFalpha, NIK, a serine/threonine kinase, becomes activated and phosphorylates IKKα and IKKβ. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKKβ autophosphorylates, which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKKβ activity can also be regulated by MEKK-1. IKK then binds to and phosphorylates IkBalpha on the two serine residues. This phosphorylation marks IkB for ubiquination and destruction by the proteosome. This free the NFkB complex and allows it to translocates to the nucleus.
      References
      Product Information
      FormatAffinity Purified
      Control
      • MCF-7 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.150 mM NaCl and 0.05% sodium azide.
      Applications
      ApplicationThis Anti-IKKβ Antibody is validated for use in WB for the detection of IKKβ.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis (SNAP ID Technique): 2-10 µg/mL from a previous lot worked in western blot using the SNAP ID technique.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to IKKβ.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes IKKβ.
      Species Reactivity
      • Human
      • Canine
      • Chicken
      • Bovine
      • Feline
      • Mouse
      Species Reactivity NoteProven to react with human. Predicted to react with cat, dog, chicken, cow, and mouse based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryNFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine)
      Gene Symbol
      • EC 2.7.11.10
      • FLJ40509
      • IKK-B
      • IKK-beta
      • IKK2
      • IKKB
      • IkBKB
      • MGC131801
      • NFKBIKB
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
      CATALYTIC ACTIVITY: ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].
      SUBUNIT STRUCTURE: Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Part of a 70-90 kDa complex at least consisting of CHUK/IKKA, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Interacts with SQSTM1 through PRKCZ or PRKCI. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6. May interact with MAVS/IPS1. Interacts with NALP2. Interacts with TICAM1. Interacts with Yersinia yopJ. Interacts with FAF1; the interaction disrupts the IKK complex formation. Interacts with ATM. Part of a ternary complex consisting of TANK, IKBKB and IKBKG. Interacts with NIBP; the interaction is direct.
      SUBCELLULAR LOCATIION: Cytoplasm.
      TISSUE SPECIFICITY: Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
      PTM: Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
      Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, which blocks the I-kappa-B signaling pathway.
      Ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues.
      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.
      Molecular Weight~85-90 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in MCF-7 cell lysate.
      Western Blot Analysis: : 1-4 µg/mL of this antibody detected IKKβ in 10 µg of MCF-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at 2-8°C for 1 year from date of receipt.
      Packaging Information
      Material Size150 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies