06-1089 | Anti-IL-12R-beta-1 Antibody

06-1089
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1089
      DescriptionAnti-IL-12R-beta-1 Antibody
      Alternate Names
      • interleukin 12 receptor, beta 1
      • interleukin-12 receptor beta-1 chain
      • IL-12R-beta-1
      • IL-12R subunit beta-1
      • interleukin-12 receptor subunit beta-1
      • IL-12 receptor beta component
      • CD212 antigen
      • IL-12 receptor subunit beta-1
      Background InformationInterleukin 12 receptor beta 1 (IL-12R-beta-1) is a low affinity receptor for IL-12. IL-12R-beta-1 is thought to combine with IL-12R-beta-2 to form a high affinity receptor for IL-12. It can also associate with IL-23R to form the receptor for Interleukin 23. IL-12R-beta-1, in the IL-23R form, may be involved in IL23 signal transduction through the launch of the Jak-Stat signaling cascade and is also thought to play a role in IL-12 transduction. IL-12R-beta-1 deficiency is often linked to severe tuberculosis.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Jurkat cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect IL-12R-beta-1 using this Anti-IL-12R-beta-1 Antibody validated for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: 1:300 dilution from a previous lot detected IL-12R-beta-1 in normal human spleen tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human IL-12R-beta-1 at the cytoplasmic domain.
      EpitopeCytoplasmic domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes IL-12R-beta-1 at the cytoplasmic domain.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The lack of expression of this gene was found to result in the immunodeficiency of patients with severe mycobacterial and Salmonella infections. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq].
      Gene Symbol
      • IL-12RB1
      • CD212
      • IL-12R-BETA1
      • IL12RB
      • IL12RB1
      • IL12R
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade. Ref.9

      SUBUNIT STRUCTURE: Dimer or oligomer; disulfide-linked. Interacts with IL12RB2 to form the high affinity IL12 receptor. Heterodimer with IL23R; in presence of IL23. The heterodimer forms the IL23 receptor.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

      DOMAIN: The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

      The box 1 motif is required for JAK interaction and/or activation.

      INVOLVEMENT IN DISEASE: Defects in IL12RB1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.

      SEQUENCE SIMLARITIES: Belongs to the type I cytokine receptor family. Type 2 subfamily.

      Contains 5 fibronectin type-III domains.
      Molecular Weight ~110 kDa observed. (Stern, A., et al. (1996). Life Sciences. 58(8):639-654.)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Jurkat cell lysate.

      Western Blot Analysis: 0.025 µg/mL of this antibody detected IL-12R-beta-1 on 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications