06-1084 | Anti-IL-4R (CD124) Antibody, cytoplasmic

06-1084
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, ChpELISA, ICC, IH(P), FCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1084
      DescriptionAnti-IL-4R (CD124) Antibody, cytoplasmic
      Alternate Names
      • CD124 antigen
      • interleukin 4 receptor
      • interleukin 4 receptor alpha chain
      • interleukin-4 receptor alpha chain
      Background InformationInterleukin 4 receptor (IL-4R) is a type I cytokine receptor that can bind interleukin 4 and interleukin 13 to regulate IgE antibody production in B cells. In T cells, IL-4R can also bind interleukin 4 to promote differentiation of Th2 cells. Soluble IL-4R can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human tonsil tissue, human B and T lymphocytes
      PresentationPurified rabbit polyclonal in PBS with 0.09% sodium azide.
      Applications
      ApplicationThis Anti-IL-4R (CD124) Antibody, cytoplasmic is validated for use in ELISA, IC, IH(P), FC for the detection of IL-4R (CD124).
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: 5 µg from a previous lot detected IL-4R in human PBMCs.

      Immunocytochemsitry: A previous lot of this antibody positively stained human B and T lymphocytes.

      ELISA: A previous lot of this antibody was shown to work in ELISA by an independent laboratory.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human IL-4R at and around the cytoplasmic domain.
      EpitopeCytoplasmic Domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the cyoplasmic domain of IL-4R.
      Species Reactivity
      • Human
      • Chimpanzee
      Species Reactivity NoteProven to react with human. Predicted to react with Chimpanzee based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Two transcript variants encoding different isoforms, a membrane-bound and a soluble form, have been found for this gene. [provided by RefSeq]
      Gene Symbol
      • IL4R
      • CD124
      • IL-4R-alpha
      • IL4RA
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammmation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.

      Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.

      SUBUNIT STRUCTURE: The functional IL4 receptor is formed by initial binding of IL4 to IL4R. Subsequent recruitment to the complex of the common gamma chain, in immune cells, creates a type I receptor and, in non-immune cells, of IL13RA1 forms a type II receptor. IL4R can also interact with the IL13/IL13RA1 complex to form a similar type II receptor. Interacts with PIK3C3 By similarity. Interacts with the SH2-containing phosphatases, PTPN6/SHIP1, PTPN11/SHIP2 and INPP5D/SHIP By similarity. Interacts with JAK1 through a Box 1-containing region. In Th1 cells, binds the N-terminal 50 amino acids of SOCS5, which inhibits JAK1 interaction, STAT6 activation and Th2 cell differentiation. SOCS5 interaction is independent of tyrosine phosphorylation By similarity.

      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein.

      Isoform 2: Secreted.

      TISSUE SPECIFICTY: Both isoform 1 and isoform 2 are highly expressed in activated T-cells.

      DOMAIN: The extracellular domain represents the IL4 binding protein (IL4BP).

      The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

      The box 1 motif is required for JAK interaction and/or activation.

      Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

      PTM: On IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction.

      The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase.

      POLYMORPHISM: Allelic variants in IL4RA are associated with a susceptibility to atopy, an immunological condition that can lead to clinical symptoms such as allergic rhinitis, sinusitis, asthma and eczema.

      SEQUENCE SIMILARITIES: Belongs to the type I cytokine receptor family. Type 4 subfamily.

      Contains 1 fibronectin type-III domain.
      Molecular Weight~89 kDa.
      2 isoforms produced by alternative splicing: isoform 1 ~90 kDa and isoform 2 ~25 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by immunohistochemistry in human tonsil tissue.

      Immunohistochemistry (paraffin) Analysis: A 1:100 dilution of this antibody positively stained restricted lymphocytes in the corona and mantle cell regions of the tonsil follicles.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications