06-1085-I | Anti-IL-7Ra (CD127), extracellular Antibody

06-1085-I
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, FCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1085-I
      DescriptionAnti-IL-7Ra (CD127), extracellular Antibody
      Alternate Names
      • Interleukin-7 receptor subunit alpha
      • IL-7 receptor subunit alpha
      • IL-7R subunit alpha
      • IL-7R-alpha
      • IL-7RA
      • CDw127
      • CD127
      Background InformationThe R34-34 antibody is specific for human CD127, a cell surface protein also known as the Interleukin-7 Receptor alpha chain, or IL-7R alpha. CD127 is typically expressed at the cell surface as a heterodimer with the common gamma chain (CD132). This complex acts as the functional receptor for IL-7, a cytokine important in T and B cell development, and in mature T cell homeostasis. A second cytokine known as Thymic Stromal Lymphopoietin (TSLP) also binds to a receptor complex of CD127 and the TSLPR chain to trigger activation of dendritic cells, and is involved in B cell development, allergy and autoimmunity. The R34-34 antibody may be used as a phenotypic marker for CD127 on immature B cells, on subsets of thymocytes which are double negative (CD4-CD8- ) or single positive (CD4+ or CD8+), and at low levels on mature, peripheral T cells. CD127 is a key marker, when used in combination with CD4 and CD25, to distinguish Treg and effector/memory Treg populations known as T(REM), whereas certain populations of Foxp3+ /CD25+ T cells lacking CD127 have been associated with impaired suppressive function (Bikker et al. 2012. Rheumatology, 51: 996-1005)
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-IL-7Ra (CD127), extracellular Antibody is validated for use in Western Blotting and Flow Cytometry for the detection of IL-7Ra (CD127), extracellular.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesWestern Blotting Analysis: 2.0 µg/mL from a representative lot detected IL-7Ra (CD127) in 10 µg of human spleen tissue lysate.
      Flow Cytometry Analysis: 0.25 µg of this antibody from a representative lot detected IL-7Ra (CD127) in 1X10E6 human peripheral blood mononuclear cells (PBMCs).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the extracellular domain of human IL-7Ra (CD127).
      EpitopeExtracellular domain
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IL7R
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~70 kDa observed. This protien is highly and variably glycosylated, and may vary from the calculated molecular weight of ~52kDa (Vranjkovi, A., et al., International Immunology, Vol. 19, No. 12, pp. 1329–1339). Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected IL-7Ra (CD127) in 10 µg of K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications