05-940 | Anti-INCENP Antibody, clone 58-217

05-940
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-940
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-INCENP Antibody, clone 58-217
      References
      Product Information
      FormatPurified
      Applications
      ApplicationDetect INCENP with Anti-INCENP Antibody, clone 58-217 (Mouse Monoclonal Antibody), that has been shown to work in WB.
      Key Applications
      • Western Blotting
      Biological Information
      Immunogen6His tagged fusion protein corresponding to residues 231-398 of human Inner Centromere Protein (INCENP). Clone 58-217.
      Cloneclone 58-217
      HostMouse
      SpecificityINCENP
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteNOT mouse or rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryIn mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).[supplied by OMIM]
      Gene Symbol
      • INCENP
      • FLJ31633
      • MGC111393
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9NQS7 # Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules.
      SIZE: 923 amino acids; 106114 Da
      SUBUNIT: Homodimer or heterodimer. Interacts with H2AFZ (By similarity). Interacts with CBX3. Interacts with tubulin beta chain. Interacts with AURKB and AURKC. Component of the CPC at least composed of BIRC5/survivin, CDCA8/borealin, INCENP and AURKB/Aurora-B. Interacts with EVI5.
      SUBCELLULAR LOCATION: Centromere. Spindle. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
      Molecular Weight135-155kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-INCENP, clone 58-217 (mouse monoclonal IgG1) 2994755
      Anti-INCENP, clone 58-217 (mouse monoclonal IgG1) 2903458
      Anti-INCENP, clone 58-217 - 30531 30531

      References

      Reference overviewApplicationSpeciesPub Med ID
      Interphase cytofission maintains genomic integrity of human cells after failed cytokinesis.
      Choudhary, A; Lera, RF; Martowicz, ML; Oxendine, K; Laffin, JJ; Weaver, BA; Burkard, ME
      Proceedings of the National Academy of Sciences of the United States of America  110  13026-31  2013

      Show Abstract
      Immunofluorescence23878225 23878225
      Human RASSF7 regulates the microtubule cytoskeleton and is required for spindle formation, Aurora B activation and chromosomal congression during mitosis.
      Recino, A; Sherwood, V; Flaxman, A; Cooper, WN; Latif, F; Ward, A; Chalmers, AD
      The Biochemical journal  430  207-13  2010

      Show Abstract Full Text Article
      ImmunofluorescenceHuman20629633 20629633
      Relocation of Aurora B from centromeres to the central spindle at the metaphase to anaphase transition requires MKlp2.
      Gruneberg, Ulrike, et al.
      J. Cell Biol., 166: 167-72 (2004)  2004

      Show Abstract
      15263015 15263015
      Aurora-B phosphorylation in vitro identifies a residue of survivin that is essential for its localization and binding to inner centromere protein (INCENP) in vivo.
      Wheatley, Sally P, et al.
      J. Biol. Chem., 279: 5655-60 (2004)  2004

      Show Abstract
      14610074 14610074
      Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis.
      Honda, Reiko, et al.
      Mol. Biol. Cell, 14: 3325-41 (2003)  2003

      Show Abstract
      12925766 12925766