05-430 | Anti-INK4b (p15) Antibody

05-430
400 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WBMCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number05-430
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-INK4b (p15) Antibody
      References
      Product Information
      FormatCulture Supernatant
      Presentationculture supernatant with 0.05% sodium azide
      Applications
      ApplicationDetect INK4b (p15) with Anti-INK4b (p15) Antibody (Mouse Monoclonal Antibody), that has been shown to work in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: 100 µL from a representative lot immunoprecipitated INK4b (p15) from 1 mg of HeLa cell lysate.
      Western Blotting Analysis: A representative lot detected an upregulated INK4b level in a K-Raslox MEF cell line DU315-6 following Ras-knockdown induction by a 12-day 4OHT treatment (Azrak, S.S., et al. (2013). BMC Genomics. 14:731).
      Western Blotting Analysis: A representative lot detected INK4b in human HNSCC FaDU and Detroit 562 cells (Sikka, A., et al. (2012). Cell Cycle. 11(7):1374-182).
      Western Blotting Analysis: A representative lot detected INK4b in human HepG2 and rat FTO-2B, but not mouse Hep-55.1C, hepatoma cell lysates (Buenemann, C.L., et al. (2001). Carcinogenesis. 22(3):447-452).
      Biological Information
      ImmunogenFull length fusion protein correspond-ing to human p15 sequence
      HostMouse
      Specificityp15
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.
      Gene Symbol
      • CDKN2B
      • TP15
      • p15INK4b
      • p14-INK4b
      • MTS2
      • p15-INK4b
      • p14_INK4B
      • p15INK4B
      • INK4B
      • P15
      • CDK4I
      • p15_INK4B
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42772 # Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.
      SIZE: 138 amino acids; 14722 Da
      SUBUNIT: Heterodimer of CDKN2B with CDK4 or CDK6.
      DISEASE:SwissProt: P42772 # Defects in CDKN2B are involved in tumor formation.
      SIMILARITY: SwissProt: P42772 ## Belongs to the CDKN2 cyclin-dependent kinase inhibitor family. & Contains 4 ANK repeats.
      Molecular Weight15 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:250-100 dilution of this antibody detected INK4b (p15) in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size400 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-INK4b (p15) (culture supernatant) - 2152219 2152219
      Anti-INK4b (p15) (culture supernatant) - 2457107 2457107
      Anti-INK4b (p15) (culture supernatant) - 2165914 2165914
      Anti-INK4b (p15) (culture supernatant) - 2194155 2194155
      Anti-INK4b (p15) (culture supernatant) - 2218831 2218831
      Anti-INK4b (p15) (culture supernatant) - 2253867 2253867
      Anti-INK4b (p15) (culture supernatant) - DAM1785839 DAM1785839
      Anti-INK4b (p15) - 0611045857 0611045857
      Anti-INK4b (p15) - 16224 16224
      Anti-INK4b (p15) - 2059383 2059383

      References

      Reference overviewApplicationPub Med ID
      Reversible, interrelated mRNA and miRNA expression patterns in the transcriptome of Rasless fibroblasts: functional and mechanistic implications.
      Azrak, SS; Ginel-Picardo, A; Drosten, M; Barbacid, M; Santos, E
      BMC genomics  14  731  2013

      Show Abstract
      Western Blotting24156637 24156637
      Metformin suppresses growth of human head and neck squamous cell carcinoma via global inhibition of protein translation.
      Sikka, A; Kaur, M; Agarwal, C; Deep, G; Agarwal, R
      Cell cycle (Georgetown, Tex.)  11  1374-82  2012

      Show Abstract
      22421144 22421144
      Bcl-2 activates a programme of premature senescence in human carcinoma cells.
      Elvira Crescenzi, Giuseppe Palumbo, Hugh J M Brady
      The Biochemical journal  375  263-74  2003

      Show Abstract Full Text Article
      12871207 12871207
      Transforming growth factor-beta1-induced Smad signaling, cell-cycle arrest and apoptosis in hepatoma cells.
      C L Buenemann, C Willy, A Buchmann, A Schmiechen, M Schwarz, C L Buenemann, C Willy, A Buchmann, A Schmiechen, M Schwarz
      Carcinogenesis  22  447-52  2001

      Show Abstract
      11238185 11238185
      The subcellular locations of p15(Ink4b) and p27(Kip1) coordinate their inhibitory interactions with cdk4 and cdk2
      Reynisdóttir, I and Massagué, J
      Genes Dev, 11:492-503 (1997)  1997

      9042862 9042862
      Evidence for different modes of action of cyclin-dependent kinase inhibitors: p15 and p16 bind to kinases, p21 and p27 bind to cyclins
      Hall, M, et al
      Oncogene, 11:1581-8 (1995)  1995

      7478582 7478582
      p15INK4B is a potential effector of TGF-beta-induced cell cycle arrest
      Hannon, G J and Beach, D
      Nature, 371:257-61 (1994)  1994

      8078588 8078588

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies