05-784R | Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal

05-784R
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, HWB, IPRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-784R
      DescriptionAnti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal
      Alternate Names
      • insulin receptor substrate 1
      Background InformationIRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. It is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer.
      References
      Product Information
      FormatPurified
      Control
      • 3T3/A31, 3T3/L1, L6, or MCF7 cell lysates
      PresentationPurified Rabbit Monoclonal IgG Supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide and 40% glycerol.
      Applications
      ApplicationDetect IRS1 using this Anti-IRS1 Antibody, clone 58-10C-31 validated for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesWestern Blot (SNAP ID) Analysis: 0.1 µg/mL from a previous lot detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6 and MCF7 cell lysates.

      Immunoprecipitation Analysis: 1 µg from a previous lot immunoprecipitated IRS-1 from 100 µg of MCF7 cell lysate. Arrow indicates IRS-1 (~160 kDa). There is cross reactivity of the detection antibody with the heavy chaing of Rabbit IgG as shown at ~50 kDa.
      Biological Information
      ImmunogenKLH- conjugated - linear peptide corresponding to the C-terminus of rat IRS1.
      EpitopeC-terminus
      Clone58-10C-31
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityAntibody recognizes the C-terminus of IRS1.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      • Human
      Species Reactivity NoteReacts with mouse, rat and human.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IRS1
      • IRS-1
      • HIRS-1
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. Ref.3

      SUBUNIT STRUCTURE: Interacts with SOCS7 By similarity. Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1.

      PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-307 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor, and Ser-789 phosphorylation is increased in the liver of insulin-resistant rats.

      Phosphorylation of Tyr-895 is required for GRB2-binding.

      SEQUENCE SIMILARITIES: Contains 1 IRS-type PTB domain.

      Contains 1 PH domain.
      Molecular Weight ~ 160 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in 3T3/A31, 3T3/L1, L6 or MCF7 cell lysates.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6, or MCF7 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-IRS1, clone 58-10C-31 2475663
      Anti-IRS1, clone 58-10C-31 - 2459055 2459055
      Anti-IRS1, clone 58-10C-31 - 2009444 2009444
      Anti-IRS1, clone 58-10C-31 - 2150090 2150090
      Anti-IRS1, clone 58-10C-31 - 2207201 2207201
      Anti-IRS1, clone 58-10C-31 - 2299140 2299140
      Anti-IRS1, clone 58-10C-31 - 2302240 2302240
      Anti-IRS1, clone 58-10C-31 - 2377415 2377415
      Anti-IRS1, clone 58-10C-31 - 2563501 2563501
      Anti-IRS1, clone 58-10C-31 - NG1861488 NG1861488

      Brochure

      Title
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

      Technical Info

      Title
      Protein Blotting Handbook: 6th Edition