04-784 | Anti-IRS1 Antibody, clone AW58 | 04-784

04-784
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-784
      Replaces05-784
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-IRS1 Antibody, clone AW58 | 04-784
      Alternate Names
      • insulin receptor substrate 1
      Background InformationInsulin Receptor substrate proteins are the effectors of both Insulin and IGF-initiated signaling. They share PH and PTB domains near their n-termini, and multiple Tyr phosphorylation motifs in their c-terminal regions. Proteins which bind to tyrosine-phosphorylated IRS-proteins include PI3 Kinase p85, GRB2, SHP2, Nck, Crk, and Fyn. IRS1 appears to be principally involved in IGF-signaling and cytoskeletal growth. IRS2 appears to be the critical mediator of Insulin signaling, as genetic ablation results in type II diabetes. IRS3 is expressed primarily in adipocytes and is an unusually potent activator of PI3 Kinase. IRS4 lacks the tyrosine residues which in the other IRS-proteins binds to SHP2.
      References
      Product Information
      FormatPurified
      PresentationCultured supernantant in 0.05% sodium azide
      Applications
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoblot Analysis: 1:1,000 to 1:4,000 dilutions of this lot detected IRS1 in RIPA lysates from 3T3/A31 mouse fibroblasts.

      Immunoprecipitation: 5-10μl of this lot immunoprecipitated IRS1 from 500μg of 3T3/A31 RIPA lysate.

      Biological Information
      ImmunogenPeptide corresponding to carboxy-terminal 14 amino acids (C-YASINFQKQPEDRQ) of rat liver IRS1
      CloneAW58
      HostRabbit
      SpecificityIRS1
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IRS1
      • IRS-1
      • HIRS-1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35568 # May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
      SIZE: 1242 amino acids; 131591 Da
      SUBUNIT: Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. Binds to UBTF and PIK3CA in nuclear extracts (By similarity). Interacts with SOCS7.
      PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity). & Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity).DISEASE:SwissProt: P35568 # Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
      SIMILARITY: SwissProt: P35568 ## Contains 1 IRS-type PTB domain. & Contains 1 PH domain.
      Molecular WeightMr ~145-165kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on in RIPA lysates from 3T3/A31 mouse fibroblasts
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C from date of shipment
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-IRS1, clone AW58 - 2391056 2391056
      Anti-IRS1, clone AW58 - 2299157 2299157
      Anti-IRS1, clone AW58 - DAM1561695 DAM1561695
      Anti-IRS1, clone AW58 -2571201 2571201
      Anti-IRS1, clone AW58 -2677128 2677128
      Anti-IRS1, clone AW58 -2787726 2787726

      References

      Reference overviewApplicationPub Med ID
      Mammalian target of rapamycin pathway regulates insulin signaling via subcellular redistribution of insulin receptor substrate 1 and integrates nutritional signals and metabolic signals of insulin
      Takano, A., et al
      Mol Cell Biol, 21:5050-62 (2001)  2001

      Immunoblotting (Western), Immunoprecipitation11438661 11438661
      Components of signaling pathways for insulin and insulin-like growth factor-I in muscle myoblasts and myotubes.
      Lamphere, L and Lienhard, G E
      Endocrinology, 131: 2196-202 (1992)  1992

      1385098 1385098

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies