06-526 | Anti-IRS1 Antibody, pre-CT

06-526
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-526
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-IRS1 Antibody, pre-CT
      References
      Product Information
      FormatPurified
      Control
      • Positive Control Included: 3T3 Cell Lysate (12-305)
      PresentationImmunoaffinity Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide, and 5mg/ml BSA before the addition of glycerol to 30%
      Applications
      ApplicationAnti-IRS1 Antibody, pre-CT is an antibody against IRS1 for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenKLH-linked, precarboxy-terminal 16 residue peptide corresponding to amino acid residues 1220-1235 of rat liver IRS-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes IRS1; does not recognize IRS2.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IRS1
      • IRS-1
      • HIRS-1
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35568 # May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
      SIZE: 1242 amino acids; 131591 Da
      SUBUNIT: Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. Binds to UBTF and PIK3CA in nuclear extracts (By similarity). Interacts with SOCS7.
      PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity). & Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity).DISEASE:SwissProt: P35568 # Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
      SIMILARITY: SwissProt: P35568 ## Contains 1 IRS-type PTB domain. & Contains 1 PH domain.
      Molecular Weight160-185 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on a 3T3/A31 RIPA cell lysate or in a L6 RIPA cell lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-IRS-1, pre CT 2474890
      Anti-IRS1, pre-CT - 14361 14361
      Anti-IRS1, pre-CT - 21114 21114
      Anti-IRS1, pre-CT - 25289 25289

      References

      Reference overviewPub Med ID
      Insulin receptor and IRS-1 co-immunoprecipitation with SOCS-3, and IKKα/β phosphorylation are increased in obese Zucker rat skeletal muscle.
      Zolotnik, IA; Figueroa, TY; Yaspelkis, BB
      Life sciences  91  816-22  2012

      Show Abstract
      22982470 22982470
      Leptin induces mitogenic effect on human choriocarcinoma cell line (JAr) via MAP kinase activation in a glucose-dependent fashion.
      G Bifulco, A Trencia, M Caruso, G A Tommaselli, C Miele, C di Carlo, F Beguinot, C Nappi, G Bifulco, A Trencia, M Caruso, G A Tommaselli, C Miele, C di Carlo, F Beguinot, C Nappi
      Placenta  24  385-91  2003

      Show Abstract
      12657513 12657513
      Phosphatidylinositol 3'-kinase and p70s6k are required for insulin but not bisperoxovanadium 1,10-phenanthroline (bpV(phen)) inhibition of insulin-like growth factor binding protein gene expression. Evidence for MEK-independent activation of mitogen-activated protein kinase by bpV(phen).
      Band, C J and Posner, B I
      J. Biol. Chem., 272: 138-45 (1997)  1997

      Show Abstract
      8995239 8995239
      The type I interferon receptor mediates tyrosine phosphorylation of insulin receptor substrate 2.
      Platanias, L C, et al.
      J. Biol. Chem., 271: 278-82 (1996)  1996

      Show Abstract
      8550573 8550573

      Related Products & Applications

      Related Products

      Included Positive Control

      Catalogue Number Description  
      12-305 3T3/A31 Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies