09-412 | Anti-ISG15/UCRP Antibody

100 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbPurifiedPolyclonal Antibody
      Catalogue Number09-412
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-ISG15/UCRP Antibody
      OverviewISG15 (also called ubiquitin cross-reactive protein (UCRP) due to its cross-reactivity with ubiquitin antibodies) was originally identified as an interferon stimulated gene (ISG) whose expression is highly induced upon interferon treatment. ISG15 contains two ubiquitin homology domains in tandem and shows ~30% identity to ubiquitin. Unlike ubiquitin, that is almost 100% homologous across species, ISG15 is only conserved in dispersed regions. Homology among the five identified mammalian ISG15 proteins is only about 47%. Like other Ubls, ISG15 is synthesized as a 17-kDa precursor form and processed to 15- kDa protein by a specific protease in order to expose di-glycine residues at the carboxyl terminal which is critical for subsequent conjugation to target proteins.
      Product Information
      Presentation100 µL of purified by salt precipitation and peptide immunochromatography in PBS containing 0.01 M sodium azide. Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).
      ApplicationDetect ISG15/UCRP using this Anti-ISG15/UCRP Antibody validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPeptide corresponding to residues 85-101 of human ISG15.
      SpecificityAntibody 09-412 recognizes several members of the ubiquitin-like (Ubl) family including ubiquitin, SUMO-1, SUMO-2, SUMO-3, Nedd8, FAT10, Ubl5, Urm1 and Fub1. This antibody shows no cross-reactivity with any of these Ubls in recombinant form upon Western blotting under the conditions used.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryG1P2 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA; MIM 147660) and -beta (IFNB; MIM 147640) (Zhao et al., 2005 [PubMed 16009940]).[supplied by OMIM]
      Gene Symbol
      • ISG15
      • UCRP
      • IFI15
      • G1P2
      Purification MethodSalt precipitation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05161 # In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy (By similarity).
      SIZE: 165 amino acids; 17888 Da
      SUBUNIT: Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interaction with influenza B NS1 protein inhibits this conjugation.
      SUBCELLULAR LOCATION: Cytoplasm. Secreted. Note=UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted.
      TISSUE SPECIFICITY: Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.
      SIMILARITY: SwissProt: P05161 ## Contains 2 ubiquitin-like domains.
      Molecular Weight18 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of shipment for up to 12 months.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-ISG15/UCRP (Rabbit polyclonal IgG) - 1948802 1948802
      Anti-ISG15/UCRP (Rabbit polyclonal IgG) - 2198965 2198965
      Anti-ISG15/UCRP - 0611045512 0611045512


      Reference overviewPub Med ID
      Antiviral actions of interferons
      Samuel, C E
      Clin Microbiol Rev, 14:778-809, table of contents (2001)  2001

      11585785 11585785
      A gene in the chromosomal region 3p21 with greatly reduced expression in lung cancer is similar to the gene for ubiquitin-activating enzyme
      Kok, K, et al
      Proc Natl Acad Sci USA, 90:6071-5 (1993)  1993

      8327486 8327486