06-1064 | Anti-ITK/EMT/TSK Antibody

06-1064
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1064
      Replaces06-546
      DescriptionAnti-ITK/EMT/TSK Antibody
      Alternate Names
      • IL2-inducible T-cell kinase
      • Kinase EMT
      • T-cell-specific kinase
      • Tyrosine-protein kinase Lyk
      • homolog of mouse T-cell itk/tsk
      • tyrosine-protein kinase ITK/TSK
      Background InformationTyrosine-protein kinase ITK/TSK (UniProt: Q08881; also known as EC:2.7.10.2, Interleukin-2-inducible T-cell kinase, IL-2-inducible T-cell kinase, Kinase EMT, T-cell-specific kinase, Tyrosine-protein kinase Lyk) is encoded by the ITK (also known as EMT, LYK) gene (Gene ID: 3702) in human. ITK/TSK is a protein tyrosine kinase that plays an essential role in regulation of the adaptive immune response. It regulates the development, function, and differentiation of conventional T-cells and non-conventional natural killer T-cells. It contains four zinc binding sites. It s N-terminal PH domain allows it to be recruited to the plasma membrane by an activated PI3 kinase. This domain also contains a proline-rich region (PRR). The adjoining SH3 domain binds to PRR. The SH2 domain is required for binding tyrosine-phosphorylated substrates and the kinase domain located in the C-terminal region is required for tyrosine phosphorylation. ITK/TSK is phosphorylated at Tyr512 in the activation loop of the kinase domain by LCK and subsequent autophosphorylation at Tyr180, which lies within the substrate-binding sequence in the SH3 domain, allows the kinase activation. Mutations in ITK gene are reported to cause lymphoproliferative syndrome 1, a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus that can lead to fatal outcome.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Jurkat cell lysates
      PresentationPurified rabbit polycloncal in buffer containing PBS with 0.05% sodium azide and 50% glycerol.
      Applications
      ApplicationThis rabbit polyclonal Anti-ITK/EMT/TSK, Cat. No. 06-1064 is a rabbit polyclonal antibody that targets protein tyrosine kinase ITK/TSK and is tested for use in Immunoprecipitation and Western blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated ITK/EMT/TSK in 500 µg of Jurkat cell lysate.
      Biological Information
      ImmunogenGST-tagged recombinant fragment cvorresponding to the first 270 amino acids from the N-terminal half of human Tyrosine-protein kinase ITK/TSK spanning the PH, SH3 and a small portion of the SH2 domains.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Itk/Emt/Tsk, Mr ~72 kDa.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to cross-react with mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ITK
      • EMT
      • LYK
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~71 kDa observed; 71.83 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected ITK/EMT/TSK in 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. For maximum recovery of the product, centrifuge the original vial and prior to removing the cap. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      06-546 Anti-Itk/Emt/Tsk Antibody Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies