05-1104 | Anti-Insulin Receptor (β-Subunit) Antibody, clone CT-3

05-1104
100 µg  
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      Overview

      Replacement Information
      Replacement Information05-1104 is a recommended replacement for MAB1139

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Mk, M, RELISA, Enzyme Assays, WB, IH(P), IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1104
      ReplacesMAB1139
      DescriptionAnti-Insulin Receptor (β-Subunit) Antibody, clone CT-3
      Background InformationThe insulin receptor is a tyrosine kinase receptor that when bound to insulin, initiates multiple signal transduction pathways, including JNK, PI 3-kinase, Akt and PKC. Pharmacological intervention of these Insulin R-dependent pathways is of great interest for the treatment of insulin resistance, obesity and diabetes. The Insulin Receptor (IR) is synthesized as a single polypeptide, which is subsequently cleaved to generate an extracellular α-chain and a transmembrane and intracellular β-chain, tethered together by disulfide bonds. The β-chain has multiple tyrosine phosphorylation sites, including three autophosphorylation sites at its activation loop. The overall structure of the IR is highly homologous to the IGF-I Receptor, except in their c-termini, where the two proteins diverge somewhat. The IR signals primarily by phosphorylating the Insulin Receptor Substrate (IRS) family of proteins, which creates docking sites for SH2-domain containing proteins. Insulin signaling is highly dependent on the PI3 Kinase pathway and Akt, which appear to mediate the functions of insulin.
      References
      Product Information
      FormatPurified
      Control
      • NIH/3T3 cell lysate
      PresentationPurified mouse monoclonal IgG1 in buffer containing 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
      Applications
      ApplicationAnti-Insulin Receptor Antibody, clone CT-3 detects level of Insulin Receptor (β-Subunit) & has been published & validated for use in ELISA, EA, WB, IH(P), IH.
      Key Applications
      • ELISA
      • Enzyme Assays
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunohistochemistry
      Application NotesImmunohistochemistry (frozen and formalin/paraffin):
      2-4ug/mL of a previous lot of this antibody was used in immunohistochemistry. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min.
      Optimal working dilutions must be determined by end user.

      ELISA: A previous lot of this antibody showed to be suitable as a capture antibody for ELISA.

      Kinase Assay: A previous lot of this antibody was used for antibody-mediated capture on microplates.

      Western Blot: A previous lot of this antibody was used at 1 ug/mL, for 2 hrs at room temperature.
      Biological Information
      ImmunogenRecombinant-fragment including the C-terminal 100 amino acids of human insulin receptor, β subunit.
      Epitope100 C-Terminal amino acids of human insulin receptor, β-subunit.
      CloneCT-3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes a protein of 95 kDa, identified as the β-subunit of insulin receptor (IR). It is directed against the C-terminal region of β-subunit and shows no cross-reaction with IGF-receptors.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Monkey
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAfter removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

      Gene Symbol
      • INSR
      • CD220
      • HHF5
      • IR
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: This receptor binds insulin and has a tyrosine-protein kinase activity. Isoform Short has a higher affinity for insulin. Mediates the metabolic functions of insulin. Binding to insulin stimulates association of the receptor with downstream mediators including IRS1 and phosphatidylinositol 3'-kinase (PI3K). Can activate PI3K either directly by binding to the p85 regulatory subunit, or indirectly via IRS1.
      CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
      ENZYME REGULATION: Autophosphorylation activates the kinase activity.
      SUBUNIT: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chains carry the kinase domain. Interacts with SORBS1 but dissociates from it following insulin stimulation. Binds SH2B2. Interacts with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. The sequences surrounding the phosphorylated NPXY motif contribute differentially to either IRS1 or SHC1 recognition. Interacts with the SH2 domains of the 85 kDa regulatory subunit of PI3K (PIK3R1) in vitro, when autophosphorylated on tyrosine residues. Interacts with SOCS7.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative
      Molecular WeightRecognizes a protein of 95 kDa, identified as the β-subunit of insulin receptor (IR).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on NIH/3T3 lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected Insulin Receptor, beta subunit on 10 μg of NIH/3T3 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C in undiluted aliquots from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Insulin Receptor (#946;-Subunit), clone CT-3 - NG1739931 NG1739931
      Anti-Insulin Receptor (#946;-Subunit), clone CT-3 - 2147094 2147094
      Anti-Insulin Receptor (#946;-Subunit), clone CT-3 - NG1815314 NG1815314
      Anti-Insulin Receptor (-Subunit), clone CT-3 - 2060505 2060505
      Anti-Insulin Receptor (-Subunit), clone CT-3 - 2377407 2377407
      Anti-Insulin Receptor (-Subunit), clone CT-3 - JBC1771125 JBC1771125
      Anti-Insulin Receptor (-Subunit), clone CT-3 - NG1577352 NG1577352
      Anti-Insulin Receptor (-Subunit), clone CT-3 - NG1660997 NG1660997
      Anti-Insulin Receptor (-Subunit), clone CT-3 - NG1846026 NG1846026
      Anti-Insulin Receptor (-Subunit), clone CT-3 - NG1850694 NG1850694

      References

      Reference overviewPub Med ID
      Insulin responsiveness in metabolic syndrome after eight weeks of cycle training.
      Stuart, CA; South, MA; Lee, ML; McCurry, MP; Howell, ME; Ramsey, MW; Stone, MH
      Medicine and science in sports and exercise  45  2021-9  2013

      Show Abstract
      23669880 23669880
      Template to improve glycemic control without reducing adiposity or dietary fat.
      Krishnapuram, R; Dhurandhar, EJ; Dubuisson, O; Kirk-Ballard, H; Bajpeyi, S; Butte, N; Sothern, MS; Larsen-Meyer, E; Chalew, S; Bennett, B; Gupta, AK; Greenway, FL; Johnson, W; Brashear, M; Reinhart, G; Rankinen, T; Bouchard, C; Cefalu, WT; Ye, J; Javier, R; Zuberi, A; Dhurandhar, NV
      American journal of physiology. Endocrinology and metabolism  300  E779-89  2011

      Show Abstract
      21266671 21266671

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies