CBL158 | Anti-Integrin β2 [CD18] Antibody

CBL158
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, WB, IHC, FUNCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL158
      ReplacesCBL514
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin β2 [CD18] Antibody
      Alternate Names
      • CD18
      References
      Product Information
      FormatPurified
      PresentationThe monoclonal is presented in phosphate buffered saline containing 15mM sodium azide, pH 7.4. We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Applications
      ApplicationAnti-Integrin β2 [CD18] Antibody detects level of Integrin β2 [CD18] & has been published & validated for use in FC, WB, IH.
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Immunohistochemistry
      • Affects Function
      Application NotesImmunohistochemistry: Identification of CD18+ leucocytes on cryostat sections

      Function Blocking: Studies of cell-cell and cell-matrix adhesion, this antibody will block cell-cell interaction

      Flow Cytometry

      Western Blot

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenLeucocytes from an LGL - type leukaemia
      CloneMEM 48
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes the CD18 molecule, a 95 kDa integrin molecule (beta 2 integrin) that forms the common beta chain of the CD11/CD18 heterodimer integrins which are expressed on all leucocytes. The beta2 integrin is the ICAM 1 receptor

      FUSION PARTNER:

      Sp 2/0 - Ag 14 myeloma cell line
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe ITGB2 protein product is the integrin beta chain beta 2. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
      Gene Symbol
      • ITGB2
      • MFI7
      • LAD
      • CD18
      • MF17
      • LCAMB
      • LFA-1
      • MAC-1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05107 # Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha- D/beta-2 is a receptor for ICAM3 and VCAM1.
      SIZE: 769 amino acids; 84782 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-2 associates with either alpha-L, alpha-M, alpha-X or alpha-D. Interacts with COPS5 and RANBP9.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      PTM: Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.DISEASE:SwissProt: P05107 # Defects in ITGB2 are the cause of leukocyte adhesion deficiency type I (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.
      SIMILARITY: SwissProt: P05107 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2o-8oC for up to 12 months from date of receipt. For long term storage aliquot antibody into small volumes and store at -20°C for up to one year.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN INTEGRIN beta2 [CD18] MONOCLONAL ANTIBODY - 2136568 2136568
      MOUSE ANTI-HUMAN INTEGRIN beta2 [CD18] -2626751 2626751
      MOUSE ANTI-HUMAN INTEGRIN beta2 [CD18] MONOCLONAL ANTIBODY 2886494
      MOUSE ANTI-HUMAN INTEGRIN beta2 [CD18] MONOCLONAL ANTIBODY 2926533
      MOUSE ANTI-HUMAN INTEGRIN beta2 [CD18] MONOCLONAL ANTIBODY - 2041551 2041551
      MOUSE ANTI-HUMAN INTEGRIN beta2 [CD18] MONOCLONAL ANTIBODY - 2274255 2274255
      MOUSE ANTI-HUMAN INTEGRIN beta2 [CD18]_2845557 2845557
      MOUSE ANTI-HUMAN INTEGRIN beta2 [CD18]_2849487 2849487

      References

      Reference overviewApplicationPub Med ID
      The role of galectin-3 in phagocytosis of Candida albicans and Candida parapsilosis by human neutrophils.
      Linden, JR; Kunkel, D; Laforce-Nesbitt, SS; Bliss, JM
      Cellular microbiology  15  1127-42  2013

      Show Abstract
      23279221 23279221
      The extracellular domain of CD11d regulates its cell surface expression.
      McKillop, WM; Barrett, JW; Pasternak, SH; Chan, BM; Dekaban, GA
      Journal of leukocyte biology  86  851-62  2009

      Show Abstract
      Western Blotting19571252 19571252
      Roles of phosphatidylinositol 3-kinase and NF-kappaB in human cytomegalovirus-mediated monocyte diapedesis and adhesion: strategy for viral persistence.
      Smith, MS; Bivins-Smith, ER; Tilley, AM; Bentz, GL; Chan, G; Minard, J; Yurochko, AD
      Journal of virology  81  7683-94  2007

      Show Abstract Full Text Article
      17507481 17507481
      Human leukocytes contain a large pool of free forms of CD18
      Drbal, K, et al
      Biochem Biophys Res Commun, 275:295-9 (2000)  2000

      10964661 10964661
      Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules
      Stefanová, I and Horejsí, V
      J Immunol, 147:1587-92 (1991)  1991

      1715364 1715364
      Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein.
      Bazil, V, et al.
      Folia Biol. (Praha), 36: 41-50 (1990)  1990

      Show Abstract
      1971601 1971601
      Structure and function of leukocyte integrins.
      Larson, R S and Springer, T A
      Immunol. Rev., 114: 181-217 (1990)  1990

      2196220 2196220

      Data Sheet

      Title
      Anti-Integrin beta2 [CD18] - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies