CBL1512 | Anti-Integrin αM [CD11b] Antibody, clone OX-42

CBL1512
500 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RFC, IHC, IH(P), IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL1512
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin αM [CD11b] Antibody, clone OX-42
      Alternate Names
      • CD11b
      Background InformationIntegrin alpha M/beta 2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR 3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha M/beta 2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
      References
      Product Information
      FormatPurified
      Control
      • Brain tissue
      PresentationThe monoclonal is presented in phosphate buffered saline containing 10mM sodium azide.
      Applications
      ApplicationDetect Integrin αM [CD11b] using this Anti-Integrin αM CD11b Antibody, clone OX-42 validated for use in FC, IH, IH(P) & IP.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      Application NotesFlow cytometry

      Immunohistochemical studies of rat fresh frozen tissue sections and paraffin-embedded tissue sections following either periodate-lysine-paraformaldehyde (PLP) fixation, or acetone. Works on very lightly PFA fixed, frozen tissues. (perfusion only 4% PFA 10-15' no post-fix).

      Immunoprecipitation: use rabbit anti-mouse or anti-mouse IgG bead for capture only. The use of protein A or protein G is not recommended.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRat peritoneal macrophages
      CloneOX-42
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes most macrophages (including resident peritoneal and activated macrophages), kupffer cells, but only about 35% of alveolar macrophages. The antibody also labels dendritic cells extensively, granulocytes and cells with the morphology of microglia in brain. The antibody precipitates three polypeptides of 150, 103 and 95 KDa. The antibody inhibits complement mediated rosettes and is probably the rat equivalent of the human receptor for IC3b called CR3.
      FUSION PARTNER: NSO myeloma cell line.
      IsotypeIgG2a
      Species Reactivity
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Some integrin alpha M proteins contain an inserted amino acid (Q, between AA positions 499 and 500) resulting from a 3bp shift in a splice acceptor that inserts the codon CAG; receptors with or without the extra amino acid are functionally indistinguishable
      Gene Symbol
      • ITGAM
      • MO1A
      • CD11b
      • MAC1A
      • CD11B
      • MAC-1
      • CR3A
      • MGC117044
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P11215 # Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
      SIZE: 1152 amino acids; 127179 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Predominantly expressed in monocytes and granulocytes.
      DOMAIN: SwissProt: P11215 The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
      SIMILARITY: Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. & Contains 1 VWFA domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-RAT CD11B MONOCLONAL ANTIBODY 2870482
      MOUSE ANTI-RAT CD11B MONOCLONAL ANTIBODY 2475753
      MOUSE ANTI-RAT CD11B MONOCLONAL ANTIBODY 2959621
      MOUSE ANTI-RAT CD11B MONOCLONAL ANTIBODY - 2120957 2120957
      MOUSE ANTI-RAT CD11B MONOCLONAL ANTIBODY - 2161124 2161124
      MOUSE ANTI-RAT CD11B MONOCLONAL ANTIBODY - 2385951 2385951
      MOUSE ANTI-RAT CD11B MONOCLONAL ANTIBODY - 2201443 2201443
      MOUSE ANTI-RAT CD11B MONOCLONAL ANTIBODY - 2291675 2291675
      MOUSE ANTI-RAT CD11B MONOCLONAL ANTIBODY -2586459 2586459
      MOUSE ANTI-RAT CD11B MONOCLONAL ANTIBODY -2603828 2603828

      References

      Reference overviewApplicationPub Med ID
      Interleukin-18 expression increases in response to neurovascular damage following soman-induced status epilepticus in rats.
      Johnson, EA; Guignet, MA; Dao, TL; Hamilton, TA; Kan, RK
      Journal of inflammation (London, England)  12  43  2015

      Show Abstract
      26203299 26203299
      Neuregulin-1 inhibits neuroinflammatory responses in a rat model of organophosphate-nerve agent-induced delayed neuronal injury.
      Li, Y; Lein, PJ; Ford, GD; Liu, C; Stovall, KC; White, TE; Bruun, DA; Tewolde, T; Gates, AS; Distel, TJ; Surles-Zeigler, MC; Ford, BD
      Journal of neuroinflammation  12  64  2015

      Show Abstract
      25880399 25880399
      Involvement of phosphatase and tensin homolog deleted from chromosome 10 in rodent model of neuropathic pain.
      Huang, SY; Sung, CS; Chen, WF; Chen, CH; Feng, CW; Yang, SN; Hung, HC; Chen, NF; Lin, PR; Chen, SC; Wang, HM; Chu, TH; Tai, MH; Wen, ZH
      Journal of neuroinflammation  12  59  2015

      Show Abstract
      25889774 25889774
      Suppression of spinal connexin 43 expression attenuates mechanical hypersensitivity in rats after an L5 spinal nerve injury.
      Xu, Q; Cheong, YK; He, SQ; Tiwari, V; Liu, J; Wang, Y; Raja, SN; Li, J; Guan, Y; Li, W
      Neuroscience letters  566  194-9  2014

      Show Abstract
      24631560 24631560
      Flexibilide obtained from cultured soft coral has anti-neuroinflammatory and analgesic effects through the upregulation of spinal transforming growth factor-β1 in neuropathic rats.
      Chen, NF; Huang, SY; Lu, CH; Chen, CL; Feng, CW; Chen, CH; Hung, HC; Lin, YY; Sung, PJ; Sung, CS; Yang, SN; Wang, HM; Chang, YC; Sheu, JH; Chen, WF; Wen, ZH
      Marine drugs  12  3792-817  2014

      Show Abstract
      24979268 24979268
      Adenosine kinase, glutamine synthetase and EAAT2 as gene therapy targets for temporal lobe epilepsy.
      Young, D; Fong, DM; Lawlor, PA; Wu, A; Mouravlev, A; McRae, M; Glass, M; Dragunow, M; During, MJ
      Gene therapy  21  1029-40  2014

      Show Abstract
      25231174 25231174
      Runx1t1 (Runt-related transcription factor 1; translocated to, 1) epigenetically regulates the proliferation and nitric oxide production of microglia.
      Baby, N; Li, Y; Ling, EA; Lu, J; Dheen, ST
      PloS one  9  e89326  2014

      Show Abstract
      24586690 24586690
      Neurochemical properties of BDNF-containing neurons projecting to rostral ventromedial medulla in the ventrolateral periaqueductal gray.
      Yin, JB; Wu, HH; Dong, YL; Zhang, T; Wang, J; Zhang, Y; Wei, YY; Lu, YC; Wu, SX; Wang, W; Li, YQ
      Frontiers in neural circuits  8  137  2014

      Show Abstract
      25477786 25477786
      Toll-like receptor 4 mediates microglial activation and production of inflammatory mediators in neonatal rat brain following hypoxia: role of TLR4 in hypoxic microglia.
      Yao, L; Kan, EM; Lu, J; Hao, A; Dheen, ST; Kaur, C; Ling, EA
      Journal of neuroinflammation  10  23  2013

      Show Abstract
      Immunohistochemistry23388509 23388509
      The response of cerebral cortex to haemorrhagic damage: experimental evidence from a penetrating injury model.
      Purushothuman, S; Marotte, L; Stowe, S; Johnstone, DM; Stone, J
      PloS one  8  e59740  2013

      Show Abstract
      23555765 23555765

      Data Sheet

      Title
      Anti-Integrin alphaM [CD11b], clone OX-42 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies