CBL1512F | Anti-Integrin αM [CD11b] Antibody, clone OX-42, FITC conjugated

CBL1512F
100 assays  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RFC, IHCMFITCMonoclonal Antibody
      Description
      Catalogue NumberCBL1512F
      ReplacesMAB1404F
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin αM [CD11b] Antibody, clone OX-42, FITC conjugated
      Alternate Names
      • CD11b
      References
      Product Information
      FormatFITC
      PresentationThe conjugate is supplied as a 100 test/1 mL vial in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. For flow cytometry we recommend using 10μL of conjugate per test.
      Applications
      ApplicationThis Anti-Integrin αM CD11b Antibody, clone OX-42, FITC conjugated is validated for use in FC, IH for the detection of Integrin αM [CD11b].
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      Application NotesFlow cytometry

      Immunohistochemical studies of rat fresh frozen tissue sections following either periodate-lysine-paraformaldehyde (PLP) fixation, or acetone. Works on very lightly PFA fixed, frozen tissues. (perfusion only 4% PFA 10-15' no post-fix).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRat peritoneal macrophages
      CloneOX-42
      HostMouse
      SpecificityThis antibody recognizes most macrophages (including resident peritoneal and activated macrophages), kupffer cells, but only about 35% of alveolar macrophages. The antibody also labels dendritic cells extensively, granulocytes and cells with the morphology of microglia in brain. The antibody precipitates three polypeptides of 150, 103 and 95 KDa. The antibody inhibits complement mediated rosettes and is probably the rat equivalent of the human receptor for IC3b called CR3.
      FUSION PARTNER: NSO myeloma cell line.
      IsotypeIgG2a
      Species Reactivity
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Some integrin alpha M proteins contain an inserted amino acid (Q, between AA positions 499 and 500) resulting from a 3bp shift in a splice acceptor that inserts the codon CAG; receptors with or without the extra amino acid are functionally indistinguishable
      Gene Symbol
      • ITGAM
      • MO1A
      • CD11b
      • MAC1A
      • CD11B
      • MAC-1
      • CR3A
      • MGC117044
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P11215 # Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
      SIZE: 1152 amino acids; 127179 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Predominantly expressed in monocytes and granulocytes.
      DOMAIN: SwissProt: P11215 The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
      SIMILARITY: Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. & Contains 1 VWFA domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C.
      Packaging Information
      Material Size100 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-RAT CD11B FITC CONJUGATED MONOCLONAL ANTIBODY - 2433550 2433550
      MOUSE ANTI-RAT CD11B FITC CONJUGATED MONOCLONAL ANTIBODY - 2111261 2111261
      MOUSE ANTI-RAT CD11B FITC CONJUGATED -2578616 2578616
      MOUSE ANTI-RAT CD11B FITC CONJUGATED -2605202 2605202
      MOUSE ANTI-RAT CD11B FITC CONJUGATED -2811631 2811631
      MOUSE ANTI-RAT CD11B FITC CONJUGATED MONOCLONAL ANTIBODY 2962876
      MOUSE ANTI-RAT CD11B FITC CONJUGATED MONOCLONAL ANTIBODY 2945416
      MOUSE ANTI-RAT CD11B FITC CONJUGATED MONOCLONAL ANTIBODY 2938807
      MOUSE ANTI-RAT CD11B FITC CONJUGATED MONOCLONAL ANTIBODY - 1998924 1998924
      MOUSE ANTI-RAT CD11B FITC CONJUGATED MONOCLONAL ANTIBODY - 2058945 2058945

      References

      Reference overviewPub Med ID
      Microglial AGE-albumin is critical in promoting alcohol-induced neurodegeneration in rats and humans.
      Byun, K; Bayarsaikhan, D; Bayarsaikhan, E; Son, M; Oh, S; Lee, J; Son, HI; Won, MH; Kim, SU; Song, BJ; Lee, B
      PloS one  9  e104699  2014

      Show Abstract
      25140518 25140518
      Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
      Whiteland, J L, et al.
      J. Histochem. Cytochem., 43: 313-20 (1995)  1995

      Show Abstract
      7868861 7868861
      Differential immunochemical markers reveal the normal distribution of brain macrophages and microglia in the developing rat brain
      Milligan C.E. et al.
      J Comparative Neurol., 314:125-135 (1991)  1991

      1797868 1797868
      Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3
      Robinson A.P. et al.
      Immunol., 57:239-247 (1986)  1986

      3512425 3512425

      Data Sheet

      Title
      Anti-Integrin AlphaM [CD11b], Clone OX-42, FITC Conjugated - Data Sheet

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      Life Science Research > Antibodies and Assays > Primary Antibodies