04-1131 | Anti-Integrin alpha4 Antibody, clone EPR1355Y, rabbit monoclonal

04-1131
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IH(P), IPRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-1131
      DescriptionAnti-Integrin alpha4 Antibody, clone EPR1355Y, rabbit monoclonal
      Alternate Names
      • 269C wild type
      • CD49 antigen-like family member D
      • CD49d antigen
      • Integrin alpha-IV
      • antigen CD49D
      • alpha-4 subunit of VLA-4 receptor
      • integrin alpha 4
      • integrin alpha-4 subunit
      Background InformationIntegrin alpha4 (CD49d, VLA-4) is a member of the integrin superfamily of adhesion receptors. Integrin alpha4 inhibits cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, where other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (Teixido, 1992). Integrin alpha4 is involved in embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (Liu, 1999).
      References
      Product Information
      FormatCulture Supernatant
      Control
      • Jurkat cell lysate
      PresentationCell culture supernatant containing Rabbit Monoclonal antibody in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect Integrin alpha4 using this Anti-Integrin alpha4 Antibody, clone EPR1355Y, Rabbit validated for use in WB, IC, IH(P) & IP.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: 1:100 - 250 dilution from a previous lot detected InImmunohistochemistry Analysis: 1:100 - 250 dilution from a previous lot detected Integrin alpha4 in human tonsil tissue.

      Immunocytochemistry Analysis: 1:50 dilution from a previous lot detected Integrin alpha4 in HeLa cell lysate.

      Immunoprecipitation: 1:40 dilution from a previous lot was used on IP.
      tegrin alpha4 in human tonsil tissue.

      Biological Information
      ImmunogenSynthetic peptide corresponding to residues near the C-terminus of human Integrin alpha4.
      EpitopeC-terminus
      CloneEPR1355Y
      HostRabbit
      SpecificityThis antibody recognizes Integrin alpha4.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CD49D
      • CD49d
      • IA4
      • MGC90518
      • VLA-4
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.

      SUBUNIT STRUCTURE: Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

      DOMAIN: The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.

      PTM: Phosphorylation on Ser-1027 inhibits PXN binding.

      SEQUENCE SIMILARITIES: Belongs to the integrin alpha chain family.

      Contains 7 FG-GAP repeats.

      SEQUENCE CAUTION: The sequence AAB59613.1 differs from that shown. Reason: Frameshift at position 13.

      The sequence CAA34852.1 differs from that shown. Reason: Frameshift at position 13.

      Molecular Weight150 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on Jurkat cell lysate.

      Western Blot Analysis; 1:1,000 - 2,000 dilution of this antibody was used to detect Integrin alpha4 in Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Integrin alpha4, clone EPR1355Y, Rabbit Monoclonal - 2116003 2116003
      Anti-Integrin alpha4, clone EPR1355Y, -2547177 2547177
      Anti-Integrin alpha4, clone EPR1355Y, Rabbit Monoclonal - 1948290 1948290
      Anti-Integrin alpha4, clone EPR1355Y, Rabbit Monoclonal - 2073061 2073061
      Anti-Integrin alpha4, clone EPR1355Y, Rabbit Monoclonal - 2508989 2508989
      Anti-Integrin alpha4, clone EPR1355Y, Rabbit Monoclonal - NG1715098 NG1715098
      Anti-Integrin alpha4, clone EPR1355Y, Rabbit Monoclonal - NG1887978 NG1887978
      Anti-Integrin alpha4, clone EPR1355Y, Rabbit Monoclonal -2506080 2506080

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies