06-1067 | Anti-Interleukin 10 Receptor Alpha (IL-10RA) Antibody

06-1067
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, ChpWB, IH(P), FCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1067
      DescriptionAnti-Interleukin 10 Receptor Alpha (IL-10RA) Antibody
      Alternate Names
      • CDw210a antigen
      • interleukin 10 receptor, alpha
      • interleukin-10 receptor alpha chain
      Background InformationInterleukin 10 receptor, alpha subunit (IL-10RA or CDW210A) is a subunit for the interleukin-10 receptor and a single-pass type I membrane protein. The IL-10 receptor complex contains two subunits, the ligand binding IL10RA and the IL10RB. IL-10RA has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibit the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. IL-10RA is found in spleen, thymus, and PBMCs and is highly expressed in monocytes, B-cells, large granular lymphocytes, and T-cells. IL-10RA is weakly expressed in pancreas, skeletal muscle, brain, heart, and kidney tissues, and intermediately expressed in placenta, lung, and liver tissue.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human thymus lysate
      PresentationPurified rabbit polyclonal in PBS with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Interleukin 10 Receptor Alpha (IL-10RA) Antibody is validated for use in WB, IH(P), FC for the detection of Interleukin 10 Receptor Alpha (IL-10RA).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: 5 µg of antibody from a previous lot detected IL-10RA in human PBMC's.

      Immunohistochemistry Analysis: A 1:100 dilution from a previous lot detected IL-10RA in mouse spleen tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the cytoplasmic domain of human IL-10RA.
      EpitopeCytoplasmic Domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the cytoplasmic domain of IL-10RA.
      Species Reactivity
      • Human
      • Mouse
      • Chimpanzee
      Species Reactivity NoteDemonstrated to react with human and mouse.
      Predicted to react with chimpanzee based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq].
      Gene Symbol
      • IL10RA
      • CDW210A
      • HIL-10R
      • IL-10R-A
      • IL-10R1
      • IL10R
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Receptor for IL10; binds IL10 with a high affinity.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

      TISSUE SPECIFICTY: Spleen, thymus, and PBMC. Weak expression in pancreas, skeletal muscle, brain, heart, and kidney. Placenta, lung, and liver showed intermediate levels. Monocytes, B-cells, large granular lymphocytes, and T-cells express high levels.

      SEQUENCE SIMLARITIES: Belongs to the type II cytokine receptor family.
      Molecular Weight~63 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human thymus lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected IL-10RA on 10 µg of human thymus lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Interleukin 10 Receptor Alpha (IL-10RA) - 2430487 2430487
      Anti-Interleukin 10 Receptor Alpha (IL-10RA) - 2023190 2023190
      Anti-Interleukin 10 Receptor Alpha (IL-10RA) - 2208792 2208792
      Anti-Interleukin 10 Receptor Alpha (IL-10RA) - NG1829895 NG1829895
      Anti-Interleukin 10 Receptor Alpha (IL-10RA) - NG1947708 NG1947708
      Anti-Interleukin 10 Receptor Alpha (IL-10RA) - NRG1700055 NRG1700055

      Brochure

      Title
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.