06-546 | Anti-Itk/Emt/Tsk Antibody

06-546
250 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-546
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Itk/Emt/Tsk Antibody
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationThis Anti-Itk/Emt/Tsk Antibody is validated for use in IP, WB for the detection of Itk/Emt/Tsk.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenItk/Emt/Tsk-GST fusion protein (Itk/Emt/Tsk amino acid residues 1-270) spanning the PH, SH3 domains and a small portion of the SH2 domain
      HostRabbit
      SpecificityItk/Emt/Tsk
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.
      Gene Symbol
      • ITK
      • LYK
      • PSCTK2
      • EMT
      • MGC126258
      • MGC126257
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q08881 # Plays a role in T-cell proliferation and differentiation.
      COFACTOR: Binds 1 zinc ion per subunit (By similarity).
      SIZE: 620 amino acids; 71831 Da
      SUBCELLULAR LOCATION: Cell membrane (By similarity). Note=Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T- cells (By similarity).
      TISSUE SPECIFICITY: T-cell lines and natural killer cell lines.
      SIMILARITY: SwissProt: Q08881 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily. & Contains 1 Btk-type zinc finger. & Contains 1 PH domain. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
      Molecular Weight72 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on Jurkat cell RIPA lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Itk/Emt/Tsk - 15011 15011
      Anti-Itk/Emt/Tsk - 18781 18781
      Anti-Itk/Emt/Tsk - 27334 27334

      References

      Reference overviewPub Med ID
      In Vivo Consequences of Disrupting SH3-Mediated Interactions of the Inducible T-Cell Kinase.
      Levytskyy, RM; Hirve, N; Guimond, DM; Min, L; Andreotti, AH; Tsoukas, CD
      Journal of signal transduction  2012  694386  2012

      Show Abstract
      22649724 22649724
      A conserved motif in the ITK PH-domain is required for phosphoinositide binding and TCR signaling but dispensable for adaptor protein interactions.
      Hirve, N; Levytskyy, RM; Rigaud, S; Guimond, DM; Zal, T; Sauer, K; Tsoukas, CD
      PloS one  7  e45158  2012

      Show Abstract
      23028816 23028816
      The EMT/ITK/TSK (EMT) tyrosine kinase is activated during TCR signaling: LCK is required for optimal activation of EMT.
      Gibson, S, et al.
      J. Immunol., 156: 2716-22 (1996)  1996

      Show Abstract
      8609388 8609388
      Structure and expression of novel protein-tyrosine kinases, Emb and Emt, in hematopoietic cells.
      Yamada, N, et al.
      Biochem. Biophys. Res. Commun., 192: 231-40 (1993)  1993

      Show Abstract
      8476425 8476425

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies