06-342 | Anti-JAK3 Antibody

06-342
150 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWBRbSerumPolyclonal Antibody
      Description
      Catalogue Number06-342
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-JAK3 Antibody
      References
      Product Information
      FormatSerum
      Presentationantiserum containing 0.05% sodium azide
      Applications
      ApplicationAnti-JAK3 Antibody is an antibody against JAK3 for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      Immunogenpeptide corresponding to residues 934-952 (RDELCVAGAQLYACQDPAI) of murine JAK3. The immunizing peptide sequence shares 16/19 amino acids with rat JAK3, but contains an amino acid insertion, which may block cross-reactivity. The immunizing peptide shares 12/19 amino acids with human JAK3.
      HostRabbit
      Specificity: Recognizes murine JAK3, Mr 125kDa and JAK3 degradation products, Mr 95-120kDa. The antiserum shows very poor immunoreactivity for human JAK3. No immunoreaction was noted with immuno-precipitated mouse JAK1 and JAK2
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteThis antibody has been tested on several human cell lines including A431, Hela, Jurkat, and HEK293 cells. The antiserum shows very poor immunoreactivity for human JAK3
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryJAK3 encodes Janus kinase 3, a tyrosine kinase that belongs to the Janus family. JAK3 functions in signal transduction and interacts with members of the STAT (signal transduction and activators of transcription) family. JAK3 is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations that abrogate Janus kinase 3 function cause an autosomal SCID (severe combined immunodeficiency disease).
      Gene Symbol
      • JAK3
      • LJAK
      • L-JAK
      • JAK-3
      • JAKL
      • JAK3_HUMAN
      Purification MethodAntiserum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P52333 # Tyrosine kinase of the non-receptor type, involved in the interleukin-2 and interleukin-4 signaling pathway. Phosphorylates STAT6, IRS1, IRS2 and PI3K.
      SIZE: 1124 amino acids; 125099 Da
      SUBUNIT: Interacts with STAM2 and MYO18A (By similarity). Interacts with SHB.
      SUBCELLULAR LOCATION: Intracytoplasmic membrane; Peripheral membrane protein (By similarity). Note=Wholly intracellular, possibly membrane associated (By similarity).
      TISSUE SPECIFICITY: In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B- and M-forms are detected in cells both of hematopoietic and epithelial origins.
      DOMAIN: SwissProt: P52333 Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
      PTM: Tyrosine phosphorylated in response to IL-2 and IL-4.
      DISEASE: SwissProt: P52333 # Defects in JAK3 are a cause of autosomal recessive T-cell negative/B-cell positive type of severe combined immunodeficiency (SCID) [MIM:600802]; a condition characterized by the absence of circulating mature T-lymphocytes and NK cells, normal to elevated numbers of nonfunctional B-lymphocytes, and marked hypoplasia of lymphoid tissues.
      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain. & Contains 1 SH2 domain.
      Molecular Weight125 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on lysate from a mouse cytotoxic T lymphocyte cell line (CTLL)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size150 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-JAK3 (rabbit antiserum) - 2117164 2117164
      Anti-JAK3 (rabbit antiserum) - 2197502 2197502
      Anti-JAK3 (rabbit antiserum) - 2227215 2227215
      Anti-JAK3 (rabbit antiserum) - DAM1423772 DAM1423772
      Anti-JAK3 - 0702051804 0702051804
      Anti-JAK3 - 15358 15358
      Anti-JAK3 - 16193 16193
      Anti-JAK3 - 17985 17985
      Anti-JAK3 - 19316 19316
      Anti-JAK3 - 24131 24131

      References

      Reference overviewApplicationPub Med ID
      Analysis of histones in Xenopus laevis. I. A distinct index of enriched variants and modifications exists in each cell type and is remodeled during developmental transitions.
      Shechter, D; Nicklay, JJ; Chitta, RK; Shabanowitz, J; Hunt, DF; Allis, CD
      The Journal of biological chemistry  284  1064-74  2009

      Show Abstract
      18957438 18957438
      Regulation of JAK2 protein expression by chronic, pulsatile GH administration in vivo: a possible mechanism for ligand enhancement of signal transduction.
      Yuan Zhou, Xiaohong Wang, Jill Hadley, Seth J Corey, Regina Vasilatos-Younken
      General and comparative endocrinology  144  128-39  2005

      Show Abstract
      15993410 15993410
      Differential requirement of the cytoplasmic subregions of gamma c chain in T cell development and function.
      Tsujino, S; Di Santo, JP; Takaoka, A; McKernan, TL; Noguchi, S; Taya, C; Yonekawa, H; Saito, T; Taniguchi, T; Fujii, H
      Proceedings of the National Academy of Sciences of the United States of America  97  10514-9  2000

      Show Abstract
      10962026 10962026
      Altered interleukin-12 responsiveness in Th1 and Th2 cells is associated with the differential activation of STAT5 and STAT1.
      Gollob, J A, et al.
      Blood, 91: 1341-54 (1998)  1998

      Show Abstract
      9454765 9454765
      Involvement of proteasomes in regulating Jak-STAT pathways upon interleukin-2 stimulation
      Yu, C. L. and Burakoff, S. J.
      J Biol Chem, 272:14017-20 (1997)  1997

      Immunoblotting (Western), Immunoprecipitation9162019 9162019
      A Kaposi's sarcoma-associated herpesvirus-encoded cytokine homolog (vIL-6) activates signaling through the shared gp130 receptor subunit
      Molden, J., et al
      J Biol Chem, 272:19625-31 (1997)  1997

      Immunoprecipitation9235971 9235971
      An interleukin (IL)-13 receptor lacking the cytoplasmic domain fails to transduce IL-13-induced signals and inhibits responses to IL-4
      Orchansky, P. L., et al
      J Biol Chem, 272:22940-7 (1997)  1997

      Immunoprecipitation9278458 9278458
      Molecular characterization of specific interactions between SHP-2 phosphatase and JAK tyrosine kinases
      Yin, T., et al
      J Biol Chem, 272:1032-7 (1997)  1997

      Immunoblotting (Western), Immunoprecipitation8995399 8995399
      Shared gamma(c) subunit within the human interleukin-7 receptor complex. A molecular basis for the pathogenesis of X-linked severe combined immunodeficiency.
      Lai, S Y, et al.
      J. Clin. Invest., 99: 169-77 (1997)  1997

      Show Abstract
      Immunoblotting (Western)9005984 9005984
      Jak1 expression is required for mediating interleukin-4-induced tyrosine phosphorylation of insulin receptor substrate and Stat6 signaling molecules.
      Chen, X H, et al.
      J. Biol. Chem., 272: 6556-60 (1997)  1997

      Show Abstract
      Immunoblotting (Western)9045682 9045682

      Technical Info

      Title
      JAK/STAT Signaling Research Focus

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies