06-667 | Anti-JAK3 Antibody, CT

06-667
250 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-667
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-JAK3 Antibody, CT
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, containing 0.05% sodium azide
      Applications
      ApplicationThis Anti-JAK3 Antibody, CT is validated for use in WB for the detection of JAK3.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenSynthetic peptide GCETHAFTAHPEG KHHSLSF, representing C-terminus of human JAK3
      HostRabbit
      SpecificityJAK3
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryJAK3 encodes Janus kinase 3, a tyrosine kinase that belongs to the Janus family. JAK3 functions in signal transduction and interacts with members of the STAT (signal transduction and activators of transcription) family. JAK3 is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations that abrogate Janus kinase 3 function cause an autosomal SCID (severe combined immunodeficiency disease).
      Gene Symbol
      • JAK3
      • LJAK
      • L-JAK
      • JAK-3
      • JAKL
      • JAK3_HUMAN
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P52333 # Tyrosine kinase of the non-receptor type, involved in the interleukin-2 and interleukin-4 signaling pathway. Phosphorylates STAT6, IRS1, IRS2 and PI3K.
      SIZE: 1124 amino acids; 125099 Da
      SUBUNIT: Interacts with STAM2 and MYO18A (By similarity). Interacts with SHB.
      SUBCELLULAR LOCATION: Intracytoplasmic membrane; Peripheral membrane protein (By similarity). Note=Wholly intracellular, possibly membrane associated (By similarity).
      TISSUE SPECIFICITY: In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B- and M-forms are detected in cells both of hematopoietic and epithelial origins.
      DOMAIN: SwissProt: P52333 Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
      PTM: Tyrosine phosphorylated in response to IL-2 and IL-4.
      DISEASE: SwissProt: P52333 # Defects in JAK3 are a cause of autosomal recessive T-cell negative/B-cell positive type of severe combined immunodeficiency (SCID) [MIM:600802]; a condition characterized by the absence of circulating mature T-lymphocytes and NK cells, normal to elevated numbers of nonfunctional B-lymphocytes, and marked hypoplasia of lymphoid tissues.
      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain. & Contains 1 SH2 domain.
      Molecular Weight130 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from TF-1 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-JAK3, CT - 16297 16297

      References

      Reference overviewApplicationPub Med ID
      Ultraviolet radiation inhibits interleukin-2-induced tyrosine phosphorylation and the activation of STAT5 in T lymphocytes.
      Kulms, D; Schwarz, T
      The Journal of biological chemistry  276  12849-55  2001

      Show Abstract
      11278301 11278301
      T cell antigen receptor-mediated activation of the Ras/mitogen-activated protein kinase pathway controls interleukin 4 receptor function and type-2 helper T cell differentiation.
      Yamashita, M, et al.
      Proc. Natl. Acad. Sci. U.S.A., 96: 1024-9 (1999)  1999

      Show Abstract
      Immunoblotting (Western)9927687 9927687
      Phosphorylation and activation of the Jak-3 Janus kinase in response to interleukin-2.
      Johnston, J A, et al.
      Nature, 370: 151-3 (1994)  1994

      Show Abstract
      8022485 8022485
      Involvement of the Jak-3 Janus kinase in signalling by interleukins 2 and 4 in lymphoid and myeloid cells.
      Witthuhn, B A, et al.
      Nature, 370: 153-7 (1994)  1994

      Show Abstract
      8022486 8022486
      Molecular cloning of L-JAK, a Janus family protein-tyrosine kinase expressed in natural killer cells and activated leukocytes.
      Kawamura, M, et al.
      Proc. Natl. Acad. Sci. U.S.A., 91: 6374-8 (1994)  1994

      Show Abstract
      8022790 8022790

      Technical Info

      Title
      JAK/STAT Signaling Research Focus

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies