04-593 | Anti-JAM1 (NT) Antibody, rabbit monoclonal, clone EP1042Y

04-593
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IH(P)RbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-593
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-JAM1 (NT) Antibody, rabbit monoclonal, clone EP1042Y
      Alternate Names
      • Junctional Adhesion Molecule-1
      Background InformationJunctional Adhesion Molecules (JAMs) are components and regulators of the well-characterized epithelial and endothelial tight junction. JAM is selectively concentrated at intercellular junctions of endothelial and epithelial cells of different origins. Microscopy shows that JAM codistributes with tight junction components at the apical region of the intercellular cleft. A mAb directed to JAM (BV11) was found to inhibit spontaneous and chemokine-induced monocyte transmigration through an endothelial cell monolayer in vitro. Systemic treatment of mice with BV11 mAb blocked monocyte infiltration upon chemokine administration in subcutaneous air pouches (1). The human protein shares a highly conserved structure and sequence with the murine protein. However it is distinct in that it is constitutively expressed on circulating neutrophils, monocytes, platelets and lymphocyte subsets (2).
      References
      Product Information
      FormatPurified
      PresentationBuffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationAnti-JAM1 (N-Term) Antibody, Rabbit, clone EP1042Y detects level of JAM1 (N-Term) & has been published & validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWB: 1:2000
      IHC: 1:100 - 250 (Paraffin-embedded human liver)
      Biological Information
      ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human JAM1.
      CloneEP1042Y
      HostRabbit
      SpecificityRecognizes Human, Mouse, Rat JAM1 (N-Term).
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat (positive control: Hela cell lysate)
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryTight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established.
      Gene Symbol
      • F11R
      • JAM-A
      • PAM-1
      • JAMA
      • CD321
      • JAM
      • JAM1
      • JAM-1
      • JCAM
      • KAT
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9Y624 # Seems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation.
      SIZE: 299 amino acids; 32583 Da
      SUBUNIT: Interacts with the first PDZ domain of PARD3. The association between PARD3 and PARD6B probably disrupts this interaction (By similarity). Interacts with the ninth PDZ domain of MPDZ.
      SUBCELLULAR LOCATION: Cell junction, tight junction; Single-pass type I membrane protein. Note=Localized at tight junctions of both epithelial and endothelial cells.
      PTM: N-glycosylated.
      SIMILARITY: SwissProt: Q9Y624 ## Belongs to the immunoglobulin superfamily. & Contains 2 Ig-like V-type (immunoglobulin-like) domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20 °C. Stable for 12 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-JAM1 (N-Term.), clone EP1042Y 2470373
      Anti-JAM1 (N-Term.), clone EP1042Y - 2424479 2424479
      Anti-JAM1 (N-Term.), clone EP1042Y (Rabbit Monoclonal) - 2119997 2119997
      Anti-JAM1 (N-Term.), clone EP1042Y (Rabbit Monoclonal) - 2092643 2092643
      Anti-JAM1 (N-Term.), clone EP1042Y (Rabbit Monoclonal) - 2199588 2199588
      Anti-JAM1 (N-Term.), clone EP1042Y (Rabbit Monoclonal) - 2324523 2324523
      Anti-JAM1 (N-Term.), clone EP1042Y (Rabbit Monoclonal) - DAM1479624 DAM1479624
      Anti-JAM1 (N-Term.), clone EP1042Y - 1956569 1956569
      Anti-JAM1 (N-Term.), clone EP1042Y - DAM1644600 DAM1644600
      Anti-JAM1 (N-Term.), clone EP1042Y - DAM1658715 DAM1658715

      References

      Reference overviewApplicationPub Med ID
      Cranial irradiation alters the brain's microenvironment and permits CCR2+ macrophage infiltration.
      Morganti, JM; Jopson, TD; Liu, S; Gupta, N; Rosi, S
      PloS one  9  e93650  2014

      Show Abstract
      Western Blotting24695541 24695541
      Identification and characterisation of human Junctional Adhesion Molecule (JAM)
      Williams, L A, et al
      Mol Immunol, 36:1175-88 (1999)  1999

      10698320 10698320
      Junctional adhesion molecule, a novel member of the immunoglobulin superfamily that distributes at intercellular junctions and modulates monocyte transmigration.
      Martìn-Padura, I; Lostaglio, S; Schneemann, M; Williams, L; Romano, M; Fruscella, P; Panzeri, C; Stoppacciaro, A; Ruco, L; Villa, A; Simmons, D; Dejana, E
      The Journal of cell biology  142  117-27  1998

      Show Abstract
      9660867 9660867