07-1535 | Anti-JMJD1B (NT) Antibody

07-1535
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICC, ELISARbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1535
      DescriptionAnti-JMJD1B (NT) Antibody
      Alternate Names
      • JMJD
      • Jumonji domain-containing protein 1B
      • Nuclear protein 5qNCA
      • chromosome 5 open reading frame 7
      • jumonji domain containing 1B
      Background InformationMethylation of histones plays a critical role in regulating chromatin structure and transcription. The JMJD1 (Jumonji domain-containing protein 1) family, also known as JHDM2 (JmjC domain-containing histone demethylation protein 2) family, contains four members: hairless (HR), JMJD1A/JHDM2A, JMJD1B/JHDM2B, and JMJD1C/JHDM2C. JMJD1B has been shown to specifically demethylate mono-methyl and di-methyl histone H3 on lysine9. H3Lys9 methylation is a highly studied histone mark that represses gene expression and implicates JMJD1B as a key regulator of heterochromatin assembly. Deletions in the gene encoding JMJD1B are frequently associated with myeloid leukemias and myelodysplasias, suggesting JMJD1B also may act as a tumor suppressor.
      References
      Product Information
      FormatPurified
      Control
      • Raji nuclear extract
      PresentationPurified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
      Applications
      ApplicationUse Anti-JMJD1B (N-terminus) Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, ELISA to detect JMJD1B (N-terminus) also known as Jumonji domain-containing protein 1B.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • ELISA
      Application NotesImmunocytochemistry:
      HeLa and NIH/3T3 cells incubated with anti-JMJD1B (N-terminus) stained positively on nuclei.

      ELISA:
      This antibody was reported by an outside laboratory to be suitable for ELISA.
      Biological Information
      ImmunogenThe immunogen was a KLH-conjugated synthetic peptide corresponding to the N-terminal region of human JMJD1B.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes human JMJD1B.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, mouse. Not tested in other species.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • 5qNCA
      • C5orf7
      • EC 1.14.11.-
      • JHDM2B
      • KDM3B
      • KIAA1082
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.
      COFACTOR: Binds 1 Fe(2+) ion per subunit By similarity.
      SUBCELLULAR LOCATION: NucleusBy similarity.
      TISSUE SPECIFICTY: Ubiquitous. Highly expressed in placenta, skeletal muscle, kidney, heart and liver.
      DOMAIN: Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors By similarity.
      MISCELLANEOUS: Its gene is located in the 5q region of the genome which is deleted in del(5q) interstitial deletion, a frequent deletion found in myeloid leukemias and myelodysplasias, suggesting that it may be a good candidate for the del(5q) tumor suppressor gene.
      SEQUENCE SIMILARITIES: Belongs to the JHDM2 histone demethylase family.
      Contains 1 JmjC domain.
      Molecular Weight~190 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis:
      1:250 dilution of this antibody detected JMJD1B on 10 µg of Raji nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-JMJD1B (N-terminus) - 1959695 1959695
      Anti-JMJD1B (N-terminus) - NG1594520 NG1594520

      References

      Reference overviewPub Med ID
      Identification and characterization of TRIP8 gene in silico.
      Katoh, Masuko and Katoh, Masaru
      Int. J. Mol. Med., 12: 817-21 (2003)  2003

      Show Abstract
      14533015 14533015
      A novel nuclear protein, 5qNCA (LOC51780) is a candidate for the myeloid leukemia tumor suppressor gene on chromosome 5 band q31.
      Hu, Z, et al.
      Oncogene, 20: 6946-54 (2001)  2001

      Show Abstract
      11687974 11687974
      Transcript map and comparative analysis of the 1.5-Mb commonly deleted segment of human 5q31 in malignant myeloid diseases with a del(5q).
      Lai, F, et al.
      Genomics, 71: 235-45 (2001)  2001

      Show Abstract
      11161817 11161817
      Shotgun sequencing of the human transcriptome with ORF expressed sequence tags.
      Dias Neto, E, et al.
      Proc. Natl. Acad. Sci. U.S.A., 97: 3491-6 (2000)  2000

      Show Abstract
      10737800 10737800
      cDNA cloning and genomic structure of three genes localized to human chromosome band 5q31 encoding potential nuclear proteins.
      Lai, F, et al.
      Genomics, 70: 123-30 (2000)  2000

      Show Abstract
      11087669 11087669

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies