04-893 | Anti-JNK3/SAPK1b Antibody, clone C05T, rabbit monoclonal

04-893
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-893
      Replaces05-893
      DescriptionAnti-JNK3/SAPK1b Antibody, clone C05T, rabbit monoclonal
      Alternate Names
      • JNK3 alpha protein kinase
      • MAP kinase
      • MAP kinase p49 3F12
      • Stress-activated protein kinase JNK3
      • c-Jun N-terminal kinase 3
      • c-Jun kinase 3
      • mitogen-activated protein kinase 10
      • stress activated protein kinase JNK3
      • stress activated protein kinase beta
      Background InformationThe stress-activated protein kinase 1 family is also referred to as the jun N-terminal kinase family, in light of the substrate preference of these serine/threonine kinases. The SAPK family shares 42-45% identity in the kinase domains with the MAPK family, and the SAPKs are activated by phosphorylation of a threonine and tyrosine by MKK4 and SKK4, just as the MAP kinases are phosphorylated on a threonine and tyrosine by MEK1 and MEK2. By contrast to the mitogen-activated kinases, the stress-activated kinases are only weakly activated by mitogenic stimuli, but potently activated by stress stimuli, such as inflammatory cytokines, ischemia, chemotherapeutic agents and irradiation.
      The JNK/SAPK1 kinases, like the other MAPK-like kinases, are thought to phosphorylate multiple substrates and regulate many processes, including proliferation (in some cell types) and apoptosis. The SAPK2/3 family is most widely referred to as the p38 family. These kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. The activating kinases of SAPK2/3 are SKK2/MEK3 for SAPK2a and 2b, and MKK6 for SAPK3. The targets of the SAPK2/3 family include the MAPKAP kinases 2 and 3/3pK. In addition, SKK4 is related to this family, exhibiting 60% identity, and is activated by MKK6.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • RIPA cell lysates from A431 cells.
      PresentationCultured supernantant in 0.05% sodium azide.
      Applications
      ApplicationDetect JNK3/SAPK1b using this Anti-JNK3/SAPK1b Antibody, clone C05T validated for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis: A 1:500-1:2,000 dilution of this lot detected JNK3 in RIPA lysates from A431 cells.
      Biological Information
      ImmunogenGST fusion protein corresponding to full-length rat JNK3/ SAPK1b
      CloneC05T
      HostRabbit
      SpecificityRecognizes JNK3/SAPK1b. Does not cross react with JNK1 or JNK2.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with, and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kianse 5 can phosphorylate, and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]
      Gene Symbol
      • EC 2.7.11.24
      • FLJ12099
      • FLJ33785
      • JNK3
      • JNK3A
      • MGC50974
      • OTTHUMP00000161180
      • OTTHUMP00000161182
      • OTTHUMP00000161183
      • PRKM10
      • p493F12
      • p54bSAPK
      UniProt Number
      UniProt SummaryFUNCTION: Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity By similarity.

      Catalytic activity ATP + a protein = ADP + a phosphoprotein.

      Cofactor Magnesium By similarity.

      Enzyme regulation Activated by threonine and tyrosine phosphorylation by two dual specificity kinases, MAP2K4 and MAP2K7. MAP2K7 phosphorylates MAPK10 on Thr-221 causing a conformational change and a large increase in Vmax for the enzyme. MAP2K4 then phosphorylates Tyr-223 resulting in a further increase in Vmax. Inhibited by dual specificity phosphatases, such as DUSP1. Inhibited by HDAC9 By similarity.

      SUBUNIT: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with HDAC9 and MAPKBP1 By similarity.

      DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

      PTM:Dually phosphorylated on Thr-221 and Tyr-223, which activates the enzyme By similarity.

      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

      Contains 1 protein kinase domain.

      Molecular Weight46-54kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on RIPA lysates from A431 cells.

      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-JNK3/SAPK1b, clone C05T - 2424726 2424726
      Anti-JNK3/SAPK1b, clone C05T - 2299154 2299154
      Anti-JNK3/SAPK1b, clone C05T - 2491121 2491121
      Anti-JNK3/SAPK1b, clone C05T - DAM1561708 DAM1561708
      Anti-JNK3/SAPK1b, clone C05T -2608740 2608740
      Anti-JNK3/SAPK1b, clone C05T -2768160 2768160
      Anti-JNK3/SAPK1b, clone C05T Monoclonal Antibody 2899948

      References

      Reference overviewPub Med ID
      Small peptide inhibitor of JNK3 protects dopaminergic neurons from MPTP induced injury via inhibiting the ASK1-JNK3 signaling pathway.
      Pan, J; Li, H; Zhang, B; Xiong, R; Zhang, Y; Kang, WY; Chen, W; Zhao, ZB; Chen, SD
      PloS one  10  e0119204  2015

      Show Abstract
      25856433 25856433
      SUMOylation of the kainate receptor subunit GluK2 contributes to the activation of the MLK3-JNK3 pathway following kainate stimulation.
      Qiu-Ju Zhu,Yan Xu,Cai-Ping Du,Xiao-Yu Hou
      FEBS letters  586  2012

      Show Abstract
      22483987 22483987