07-1580 | Anti-KLF5 (Krüppel-like factor 5) Antibody

07-1580
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, HWB, ChIP, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1580
      DescriptionAnti-KLF5 (Krüppel-like factor 5) Antibody
      Alternate Names
      • BTE-binding protein 2
      • Basic transcription element-binding protein 2
      • Colon krueppel-like factor
      • GC box binding protein 2
      • GC-box-binding protein 2
      • Intestinal-enriched krueppel-like factor
      • Kruppel-like factor 5
      • Kruppel-like factor 5 (intestinal)
      • Transcription factor BTEB2
      • basic transcription element binding protein 2
      • colon kruppel-like factor
      • intestinal-enriched kruppel-like factor
      Background InformationKrüppel-like factors (KLFs) zinc finger-containing transcription factors with regulatory functions in cell growth, proliferation, differentiation, and embryogenesis. KLF5, (also known as BTEB2 and IKLF), is essential for mouse embryo development. KLF5 activates many genes during cardiovascular remodeling, including PDGFa/b, Egr-1, PAI-1, iNOS, and VEGF receptors, making KLF a potential therapeutic target for cardiovascular disease. KLF5 also plays a role in angiogenesis and in development of several tumor types including breast, prostate, bladder, colon, esophagus, and skin. KLF5 is closely related to other of the KLF family members. However, KLF5 and KLF4 exhibit opposite effects on regulation of gene transcription, despite binding to similar, if not identical, cis-acting DNA sequences. In addition, KLF4 and 5 exert contrasting effects on cell proliferation in many instances; while KLF4 is an inhibitor of cell growth, KLF5 stimulates proliferation. KLF5 protein is enriched in intestinal tissue.
      References
      Product Information
      FormatAffinity Purified
      Control
      • PC3 lysate
      PresentationAffinity purified rabbit polyclonal serum with 0.05% NaN3
      Applications
      ApplicationUse Anti-KLF5 (Krüppel-like factor 5) Antibody (Rabbit Polyclonal Antibody) validated in WB, ChIP, IHC to detect KLF5 also known as Krüppel-like factor 5.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • Immunohistochemistry
      Application NotesChromatin Immunoprecipitation:
      This antibody was demonstrated by an outside laboratory to be suitable for ChIP (2).

      Immunohistochemistry:
      This antibody was demonstrated by an outside laboratory to be suitable for immunohistochemistry using tumor tissue samples (3).
      Biological Information
      ImmunogenThe immunogen was a KLH-conjugated synthetic peptide corresponding to amino acids 95-111 of mouse/rat KLF5.
      HostRabbit
      SpecificityThis antibody recognizes human KLF5. A strong unidentified band at ~18 kDa is also detected in some cell lysates.
      Species Reactivity
      • Mouse
      • Rat
      • Human
      Species Reactivity NoteHuman and rat. Expected to react with mouse, as immunogen sequence is identical in mouse and rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CKLF
      • BTEB2
      • IKLF
      • OTTHUMP00000018502
      Purification MethodPeptide affinity chromatography
      UniProt Number
      UniProt SummaryFUNCTION: Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICITY: Expressed only in testis and placenta.

      SEQUENCE SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family.

      Contains 3 C2H2-type zinc fingers.

      Molecular Weight50 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis: :
      1:500 dilution of this lot detected KLF5 in 10 µg of PC3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-KLF5 (Kr#252;ppel-like factor 5) - 2015570 2015570
      Anti-KLF5 (Kr#252;ppel-like factor 5) - 2036696 2036696
      Anti-KLF5 (Kr#252;ppel-like factor 5) - NG1601354 NG1601354
      Anti-KLF5 (Kr#252;ppel-like factor 5) - NG1719577 NG1719577
      Anti-KLF5 (Kr#252;ppel-like factor 5) - NG1752514 NG1752514
      Anti-KLF5 (Kr#252;ppel-like factor 5) - NG1809730 NG1809730
      Anti-KLF5 (Kr#252;ppel-like factor 5) - NG1842819 NG1842819
      Anti-KLF5 (Kr#252;ppel-like factor 5) - NG1940981 NG1940981
      Anti-KLF5 (Krüppel-like factor 5) 2453129
      Anti-KLF5 (Krüppel-like factor 5) 3069727

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics