05-566 | Anti-KSR Antibody, clone C3H7

05-566
200 µg  
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      Overview

      Replacement Information
      Replacement Information04-1160 is the recommended replacement for Discontinued catalog number 05-566

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-566
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-KSR Antibody, clone C3H7
      OverviewEndogenous levels of KSR may be too low to detect by immunoblotting. Immunoprecipitation prior to immunoblotting is recommended for detection of endogenous KSR. Note levels of recombinant KSR in transfected cells may be high enough to be detected by immunoblotting.
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationDetect KSR using this Anti-KSR Antibody, clone C3H7 validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 118-248 of rat KSR.
      CloneC3H7
      HostMouse
      SpecificityKSR
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KSR1
      • RSU2
      • KSR
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q8IVT5 # Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.
      SIZE: 921 amino acids; 102032 Da
      SUBUNIT: Interacts with HSPCA/HSP90, YWHAB/14-3-3, CDC37, MAP2K/MEK, MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK, in a Ras-dependent manner (By similarity). The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane; Peripheral membrane protein (By similarity). Note=In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery (By similarity).
      PTM: Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated (By similarity).
      SIMILARITY: SwissProt: Q8IVT5 ## Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain.
      Molecular Weight120kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoprecipitation of KSR from 3T3/A31 RIPA lysate, subsequent blotting with anti-KSR.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies