06-445 | Anti-Kip1 (p27) Antibody

06-445
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, RbIHC, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-445
      Replaces04-240
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Kip1 (p27) Antibody
      Background Informationp27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4. p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.
      References
      Product Information
      FormatPurified
      Control
      • Positive Control Included: HeLa Nuclear Extract (12-309). Other controls: MCF-7 cell lysate
      PresentationProtein A Purified immunoglobulin in 0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol.
      Applications
      ApplicationAnti-Kip1 (p27) Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Kip1 (p27) & has been validated in IHC, IP & WB.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenAn 18 amino acid synthetic peptide ([C]GSVEQTPKKPGLRRRQT) linked to KLH was used to immunize rabbits
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Kip1.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      • Rabbit
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
      Gene Symbol
      • CDKN1B
      • p27Kip1
      • P27KIP1
      • MEN1B
      • MEN4
      • CDKN4
      • KIP1
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P46527 # Involved in G1 arrest. May mediate TGF beta-induced G1 arrest. Binds to and inhibits complexes formed by cyclin E-CDK2, cyclin A-CDK2, and cyclin D1-CDK4. Interaction with nucleoporin NUP50 is required for nuclear import and for degradation of phosphorylated p27Kip1 after nuclear import (By similarity).
      SIZE: 198 amino acids; 22073 Da
      SUBUNIT: Interacts with NUP50 and with UHMK1. Interacts with COPS5 subunit of COP9 signalosome complex, leading to its subsequent degradation (By similarity). Interacts with SPDYA and is found in a complex with both SPDYA and CDK2.
      SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity). Note=Nuclear and cytoplasmic in quiescent cells. Upon cell cycle progression, mostly cytoplasmic (By similarity).
      TISSUE SPECIFICITY: Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
      DOMAIN: SwissProt: P46527 A peptide sequence containing only AA 28-79 retains substantial KIP1 cyclin A/CDK2 inhibitory activity.
      PTM: Phosphorylation on Ser-10 leads to nuclear export to the cytoplasm and promotes cell cycle progression (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
      DISEASE: SwissProt: P46527 # Defects in CDKN1B are the cause of multiple endocrine neoplasia type 4 (MEN4) [MIM:610755]. Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2.
      SIMILARITY: Belongs to the CDI family.
      Molecular Weight27 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on HeLa nuclear extract or in rabbit brain nuclear extract
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Kip1 (p27) 2477967
      Anti-Kip1 (p27) (rabbit polyclonal IgG) Q2922317
      Anti-Kip1 (p27) - 13757 13757
      Anti-Kip1 (p27) - 24969 24969
      Anti-Kip1 (p27) -2514050 2514050
      Anti-Kip1 (p27) -2782643 2782643
      Anti-Kip1 (p27) -2790089 2790089

      References

      Reference overviewApplicationPub Med ID
      High-throughput screening reveals alsterpaullone, 2-cyanoethyl as a potent p27Kip1 transcriptional inhibitor.
      Walters, BJ; Lin, W; Diao, S; Brimble, M; Iconaru, LI; Dearman, J; Goktug, A; Chen, T; Zuo, J
      PloS one  9  e91173  2014

      Show Abstract
      Western Blotting24646893 24646893
      Cul4A targets p27 for degradation and regulates proliferation, cell cycle exit, and differentiation during erythropoiesis.
      Li, B; Jia, N; Kapur, R; Chun, KT
      Blood  107  4291-9  2006

      Show Abstract
      16467204 16467204
      Increased nuclear proteins in muscle satellite cells in aged animals as compared to young growing animals.
      Shuichi Machida, Frank W Booth
      Experimental gerontology  39  1521-5  2004

      Show Abstract
      15501022 15501022
      Forkhead transcription factor FoxO1 transduces insulin-like growth factor's signal to p27Kip1 in primary skeletal muscle satellite cells.
      Shuichi Machida, Espen E Spangenburg, Frank W Booth
      Journal of cellular physiology  196  523-31  2003

      Show Abstract
      12891709 12891709
      Tumor suppressor PTEN inhibits nuclear accumulation of beta-catenin and T cell/lymphoid enhancer factor 1-mediated transcriptional activation.
      Persad, S, et al.
      J. Cell Biol., 153: 1161-74 (2001)  2001

      Show Abstract
      Immunoblotting (Western)11402061 11402061
      Insulin-like growth factor-I extends in vitro replicative life span of skeletal muscle satellite cells by enhancing G1/S cell cycle progression via the activation of phosphatidylinositol 3'-kinase/Akt signaling pathway.
      Chakravarthy, MV; Abraha, TW; Schwartz, RJ; Fiorotto, ML; Booth, FW
      The Journal of biological chemistry  275  35942-52  2000

      Show Abstract
      10962000 10962000
      Cell cycle. Stifled by inhibitions.
      Peters, G
      Nature, 371: 204-5 (1994)  1994

      8078579 8078579

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      Included Positive Control

      Catalogue Number Description  
      12-309 HeLa Nuclear Extract Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies