09-820 | Anti-Klf2 Antibody

09-820
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, ChIPRbSerumPolyclonal Antibody
      Description
      Catalogue Number09-820
      DescriptionAnti-Klf2 Antibody
      Alternate Names
      • Kruppel-like factor 2 (lung)
      • Lung krueppel-like factor
      • krueppel-like factor 2
      • lung Kruppel-like zinc finger transcription factor
      • Kruppel-like factor LKLF
      Background InformationKrueppel-like factor 2 (Klf2) is a novel protein belonging to the KLF family of transcription factors, a subfamily of approximately 20 members characterized by their possession of a zinc-finger domain and functioning as transcriptional regulators for cellular differentiation and growth. Klf2 activates transcription through its binding of the beta-globin gene promoter CACCC box. Studies have shown Klf2 to have critical roles in both the function and differentiation of several cell types including; adipocytes, lung cells, T lymphocytes, and monocytes. It has also been newly identified as an endothelial transcription regulator for pro-inflammatory activation, and is a requirement for the migration of smooth muscle cells and normal vessel formation. Shear stress and pro-inflammatory cytokines are considered to be potent inhibitors of Klf2. Expression is primarily observed in lung and spleen tissue, but it has also been found in aortic tissue.
      References
      Product Information
      FormatSerum
      Control
      • Mouse embryonic stem cell chromatin extract
      PresentationRabbit polyclonal serum containing 0.05% sodium azide.
      Applications
      ApplicationUse Anti-Klf2 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Klf2 also known as Kruppel-like factor 2 (lung), Lung krueppel-like factor.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated Klf2 from 2 x 10E6 to 1 x 10E7 mouse embryonic stem cells. (Huck Ng, Genome Institute of Singapore.)
      Biological Information
      ImmunogenGST-fusion protein with histidine-tagged mouse Klf2.
      EpitopeUnknown
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Klf2
      • LKLF
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Binds to the CACCC box in the beta-globin gene promoter and activates transcription.

      SUBUNIT STRUCTURE: Interacts with WWP1 (By similarity).

      SUBCELLULAR LOCATION: Nucleus (By similarity).

      TISSUE SPECIFICTY: Predominant expression in the lungs and spleen.

      PTM: Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.

      SEQUENCE SIMILARITIES: Belongs to the krueppel C2H2-type zinc-finger protein family.

      Contains 3 C2H2-type zinc fingers.
      Molecular Weight~40 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse embryonic stem cell chromatin extract.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected Klf2 in 10 µg of mouse embryonic stem cell chromatin extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Klf2 Polyclonal Antibody 2918963
      Anti-Klf2 - 2120094 2120094
      Anti-Klf2 - 2140072 2140072
      Anti-Klf2 - 2427871 2427871
      Anti-Klf2 - 2461554 2461554
      Anti-Klf2 - 2003686 2003686
      Anti-Klf2 - 2074781 2074781
      Anti-Klf2 - 2167180 2167180
      Anti-Klf2 - 2203090 2203090
      Anti-Klf2 - 2266754 2266754

      References

      Reference overviewPub Med ID
      Klf2 is an essential factor that sustains ground state pluripotency.
      Yeo, JC; Jiang, J; Tan, ZY; Yim, GR; Ng, JH; Göke, J; Kraus, P; Liang, H; Gonzales, KA; Chong, HC; Tan, CP; Lim, YS; Tan, NS; Lufkin, T; Ng, HH
      Cell stem cell  14  864-72  2014

      Show Abstract
      24905170 24905170
      Myeloid-Specific Kruppel-Like Factor 2 Inactivation Increases Macrophage and Neutrophil Adhesion and Promotes Atherosclerosis.
      Lingrel, Jerry B, et al.
      Circulation research, (2012)  2012

      Show Abstract
      22474254 22474254