05-770 | Anti-LAT Antibody, clone 11B.12

05-770
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-770
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-LAT Antibody, clone 11B.12
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationThis Anti-LAT Antibody, clone 11B.12 is validated for use in IP, WB for the detection of LAT.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 31-233 of human LAT, cytoplasmic domain
      Cloneclone 11B.12
      HostMouse
      SpecificityLAT
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.
      Gene Symbol
      • LAT
      • LAT1
      • pp36
      • p36-38
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O43561 # Required for TCR (T-cell antigen receptor)- and pre-TCR- mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
      SIZE: 262 amino acids; 27930 Da
      SUBUNIT: When phosphorylated, interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, and LCP2. Interacts with SHB and SKAP2.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type III membrane protein. Note=Present in lipid rafts.
      TISSUE SPECIFICITY: Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
      PTM: Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. & Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
      MISCELLANEOUS: Engagement of killer inhibitory receptors (KIR) disrupts the interaction of PLCG1 with LAT and blocks target cell- induced activation of PLC, maybe by inducing the dephosphorylation of LAT.
      Molecular Weight~36/38kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on Jurkat cells and mouse thymus homogenate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-LAT, clone 11B.12 (mouse monoclonal IgG1) - 2388876 2388876
      Anti-LAT, clone 11B.12 - 26805 26805
      Anti-LAT, clone 11B.12 - DAM1780491 DAM1780491
      Anti-LAT, clone 11B.12 - DAM1780491 DAM1780491
      Anti-LAT, clone 11B.12 -2751154 2751154

      References

      Reference overviewApplicationPub Med ID
      LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation.
      Zhang, W, et al.
      Cell, 92: 83-92 (1998)  1998

      Show Abstract
      Immunoblotting (Western)9489702 9489702

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      04-466 Anti-LAT Antibody, rabbit monoclonal Show Pricing & Availability
      04-467 Anti-phospho-LAT (Tyr191) Antibody, rabbit monoclonal Show Pricing & Availability
      05-561 Anti-LAT Antibody, clone 2E9 Show Pricing & Availability
      06-807 Anti-LAT Antibody Show Pricing & Availability
      07-278 Anti-phospho-LAT (Tyr191) Antibody Show Pricing & Availability
      AB4093 Anti-LAT Antibody Show Pricing & Availability
      ABS217 Anti-phospho-LAT Antibody Show Pricing & Availability

      Included Positive Control

      Catalogue Number Description  
      12-303 Jurkat Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies