04-466 | Anti-LAT Antibody, rabbit monoclonal

04-466
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-466
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-LAT Antibody, rabbit monoclonal
      References
      Product Information
      FormatPurified
      Presentation100 μL of rabbit monoclonal IgG in 60% storage buffer (50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-LAT Antibody, Rabbit detects level of LAT & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide encompassing residues near the GRB-2 binding motif of human LAT.
      HostRabbit
      SpecificityRecognizes a synthetic peptide encompassing residues near the GRB-2 binding motif of human LAT. This antibody with react with both long and short LAT.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.
      Gene Symbol
      • LAT
      • pp36
      • LAT1
      • p36-38
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O43561 # Required for TCR (T-cell antigen receptor)- and pre-TCR- mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
      SIZE: 262 amino acids; 27930 Da
      SUBUNIT: When phosphorylated, interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, and LCP2. Interacts with SHB and SKAP2.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type III membrane protein. Note=Present in lipid rafts.
      TISSUE SPECIFICITY: Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
      PTM: Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. & Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
      MISCELLANEOUS: Engagement of killer inhibitory receptors (KIR) disrupts the interaction of PLCG1 with LAT and blocks target cell- induced activation of PLC, maybe by inducing the dephosphorylation of LAT.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-LAT (Rabbit monoclonal IgG) - DAM1794323 DAM1794323
      Anti-LAT - DAM1772445 DAM1772445
      Anti-LAT - NG1817603 NG1817603
      Anti-LAT, Rabbit Monoclonal - 0611046369 0611046369

      References

      Reference overviewApplicationPub Med ID
      Molecular cloning of the cDNA encoding pp36, a tyrosine-phosphorylated adaptor protein selectively expressed by T cells and natural killer cells
      Weber, J R, et al
      J Exp Med, 187:1157-61 (1998)  1998

      9529333 9529333
      LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation.
      Zhang, W, et al.
      Cell, 92: 83-92 (1998)  1998

      Show Abstract
      Immunoblotting (Western)9489702 9489702

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies