05-602 | Anti-LEF Antibody, clone 2D12

05-602
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-602
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-LEF Antibody, clone 2D12
      Background InformationMonoclonal Anti-LEF-1 (β-Catenin Binding Domain) recognizes the β-Catenin Binding Domain of human LEF-1 using immunoblotting (55-57 kDa). Lymphoid enhancer factor, LEF-1, is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play crucial roles in WNT/Wingless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer. LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve.
      References
      Product Information
      FormatPurified
      Control
      • Jurkat nuclear extract
      Presentation100µg of lyophilized thiophilic and size exclusion chromatography purified mouse IgG1 from 1mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 100 µL H2O (15 min, RT) for a final concentration of 1 mg/mL. Store at -20°C.
      Applications
      ApplicationAnti-LEF Antibody, clone 2D12 is a Mouse Monoclonal Antibody for detection of LEF also known as lymphoid enhancer-binding factor & has been tested in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenResidues 1-85 of human Lymphoid Enhancer Factor (LEF)
      Cloneclone 2D12
      HostMouse
      SpecificityLEF that appears as multiple polypeptides
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryLymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).[supplied by OMIM]
      Gene Symbol
      • LEF1
      • LEF-1
      • TCF1-alpha
      • TCF1ALPHA
      • DKFZp586H0919
      Purification MethodThiophilic and size exclusion chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UJU2 # Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling.
      SIZE: 399 amino acids; 44201 Da
      SUBUNIT: Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG (By similarity). Binds THOC4, MDFI and MDFIC.
      SUBCELLULAR LOCATION: Nucleus (By similarity). Note=Found in nuclear bodies upon PIASG binding (By similarity).
      TISSUE SPECIFICITY: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines.DOMAIN:SwissProt: Q9UJU2 Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
      SIMILARITY: Belongs to the TCF/LEF family. & Contains 1 HMG box DNA-binding domain.
      Molecular Weight55-57kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on Jurkat cell lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti LEF, clone 2D12 - 20331 20331
      Anti LEF, clone 2D12 - 25077 25077
      Anti LEF, clone 2D12 - 27290 27290
      Anti LEF, clone 2D12 - 33442 33442
      Anti-LEF, clone 2D12 - 0050S0202 0050S0202

      References

      Reference overviewPub Med ID
      Regulation of LEF-1/TCF transcription factors by Wnt and other signals.
      Eastman, Q and Grosschedl, R
      Curr. Opin. Cell Biol., 11: 233-40 (1999)  1999

      Show Abstract
      10209158 10209158

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies