09-058 Anti-LSD1 Antibody

09-058
100 µL  
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      Overview

      Replacement Information
      Replacement InformationMABE545 is the recommended replacement for Discontinued catalog number 09-058

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, ChIPRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number09-058
      DescriptionAnti-LSD1 Antibody
      Alternate Names
      • BRAF35-HDAC complex protein BHC110
      • FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit
      • Flavin-containing amine oxidase domain-containing protein 2
      • amine oxidase (flavin containing) domain 2
      • lysine (K)-specific demethylase 1
      • lysine-specific histone demethylase 1
      Background InformationLSD1 (lysine specific demethylase 1), also known as BHC110, is a histone demethylase that specifically demethylates Lys4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted Lys4 of histone H3, and may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 Lys4 on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. LSD1 may also demethylate Lys9 of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
      References
      Product Information
      FormatPurified
      Control
      • HeLa nuclear lysate.
      PresentationProtein A purified rabbit serum. Supplied at 1 mg/ml in PBS, 0.1% Azide
      Applications
      ApplicationAnti-LSD1 Antibody is a Rabbit Polyclonal Antibody for detection of LSD1 also known as BRAF35-HDAC complex protein BHC110, lysine (K)-specific demethylase 1 & has been validated in WB, ChIP & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunoprecipitation:
      This antibody has been demonstrated by an outside laboratory to be suitable for immunoprecipitation.

      Chromatin Immunoprecipitation:
      This antibody has been demonstrated by an outside laboratory to be suitable for ChIP3.
      Biological Information
      ImmunogenImmunogen was full length recombinant human LSD1.
      HostRabbit
      SpecificityRecognizes LSD1, Mr ~100-110 kDa
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Cross-reactivity with other species has not been determined.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternate splicing has been observed; however, not all transcript variants have been fully characterized. [provided by RefSeq]
      Gene Symbol
      • BHC110
      • KDM1
      • KIAA0601
      • LSD1
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.

      Cofactor FAD.

      SUBUNIT:Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR).

      Subcellular location Nucleus.

      SPECIFICITY: Ubiquitously expressed.

      DOMAIN: The SWIRN domain may act as an anchor site for a histone tail.

      SIMILARITY: Belongs to the flavin monoamine oxidase family.

      Contains 1 SWIRM domain.

      Molecular Weight100-110 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot Analysis.

      Western Blot Analysis:
      A 0.5 - 1 μg/mL dilution of this lot detected LSD1 in HeLa nuclear lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-LSD1 - DAM1567798DAM1567798
      Anti-LSD1 - NG1547136NG1547136

      References

      Reference overviewApplicationPub Med ID
      DNMT1 modulates gene expression without its catalytic activity partially through its interactions with histone-modifying enzymes.
      Clements, EG; Mohammad, HP; Leadem, BR; Easwaran, H; Cai, Y; Van Neste, L; Baylin, SB
      Nucleic acids research  40  4334-46  2012

      Show Abstract
      22278882 22278882
      Regulation of LSD1 histone demethylase activity by its associated factors.
      Shi, Yu-Jiang, et al.
      Mol. Cell, 19: 857-64 (2005)  2005

      16140033 16140033
      LSD1 demethylates repressive histone marks to promote androgen-receptor-dependent transcription.
      Metzger, Eric, et al.
      Nature, 437: 436-9 (2005)  2005

      Show Abstract
      16079795 16079795
      Histone demethylation mediated by the nuclear amine oxidase homolog LSD1.
      Shi, Yujiang, et al.
      Cell, 119: 941-53 (2004)  2004

      Show Abstract
      Immunoblotting (Western)15620353 15620353
      A candidate X-linked mental retardation gene is a component of a new family of histone deacetylase-containing complexes.
      Hakimi, Mohamed-Ali, et al.
      J. Biol. Chem., 278: 7234-9 (2003)  2003

      Show Abstract
      12493763 12493763

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies