05-939 | Anti-LSD1/BHC110 Antibody

05-939
100 µL  
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      Overview

      Replacement Information
      Replacement Information06-574 is a recommended replacement for CBL770

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIF, IHC, IP, WB, ChIPMAscitesMonoclonal Antibody
      Description
      Catalogue Number05-939
      ReplacesCBL770
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-LSD1/BHC110 Antibody
      Alternate Names
      • BRAF35-HDAC complex protein BHC110
      • FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit
      • Flavin-containing amine oxidase domain-containing protein 2
      • amine oxidase (flavin containing) domain 2
      • lysine (K)-specific demethylase 1
      • lysine-specific histone demethylase 1
      Background InformationHistone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and tri-methylted 'Lys-4' of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
      References
      Product Information
      FormatAscites
      Control
      • HeLa cell lysate.
      PresentationMouse monoclonal in buffer containing 0.05% sodium azide and 30% glycerol.
      Applications
      ApplicationAnti-LSD1/BHC110 Antibody is a Mouse Monoclonal Antibody for detection of LSD1/BHC110 also known as BRAF35-HDAC complex protein BHC110, lysine (K)-specific demethylase 1 & has been validated in ChIP, IF, IHC, IP & WB.
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      This antibody has been reported by an independent laboratory to immunoprecipitate LSD1 from chromatin.

      Immunoprecipitation:
      Recommended.

      Immunohistochemistry:
      This antibody has been reported by an independent laboratory to detect LSD1 using paraffin-embedded tissues. (Metzger, E., 2005)

      Immunofluorescence:
      Recommended.
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 23-138 of human LSD1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes LSD1, Mr 110 kDa.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternate splicing has been observed; however, not all transcript variants have been fully characterized.
      Gene Symbol
      • AOF2
      • KIAA0601
      • BHC110
      • LSD1
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O60341 # Histone demethylase that specifically demethylates 'Lys- 4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and tri- methylated 'Lys-4' of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate 'Lys- 9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
      COFACTOR: FAD.
      SIZE: 852 amino acids; 92903 Da
      SUBUNIT: Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, AOF2/LSD1, RCOR1/CoREST and PHF21A/BHC80. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR).
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Ubiquitously expressed.
      DOMAIN: SwissProt: O60341 The SWIRN domain may act as an anchor site for a histone tail.
      SIMILARITY: Belongs to the flavin monoamine oxidase family. & Contains 1 SWIRM domain.
      Molecular Weight110 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot.

      Western Blot Analysis:
      1:500-1:2000 dilution of this lot detected LSD1 in RIPA lysates from HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-LSD1/BHC110 2475731
      Anti-LSD1/BHC110 - 2039870 2039870
      Anti-LSD1/BHC110 - 2276335 2276335
      Anti-LSD1/BHC110 - 30522 30522
      Anti-LSD1/BHC110 - 33455 33455
      Anti-LSD1/BHC110 - JBC1361937 JBC1361937
      Anti-LSD1/BHC110 - NG1904282 NG1904282
      Anti-LSD1/BHC110 Monoclonal Antibody 2890801

      References

      Reference overviewApplicationPub Med ID
      Lysine-specific histone demethylase 1 inhibitors control breast cancer proliferation in ERα-dependent and -independent manners.
      Pollock, JA; Larrea, MD; Jasper, JS; McDonnell, DP; McCafferty, DG
      ACS chemical biology  7  1221-31  2012

      Show Abstract
      Western Blotting22533360 22533360
      Hyperglycemia induces a dynamic cooperativity of histone methylase and demethylase enzymes associated with gene-activating epigenetic marks that coexist on the lysine tail.
      Brasacchio, D; Okabe, J; Tikellis, C; Balcerczyk, A; George, P; Baker, EK; Calkin, AC; Brownlee, M; Cooper, ME; El-Osta, A
      Diabetes  58  1229-36  2009

      Show Abstract
      19208907 19208907
      LSD1 demethylates repressive histone marks to promote androgen-receptor-dependent transcription.
      Metzger, Eric, et al.
      Nature, 437: 436-9 (2005)  2005

      Show Abstract
      16079795 16079795
      Histone demethylation mediated by the nuclear amine oxidase homolog LSD1.
      Shi, Yujiang, et al.
      Cell, 119: 941-53 (2004)  2004

      Show Abstract
      Immunoblotting (Western)15620353 15620353
      A candidate X-linked mental retardation gene is a component of a new family of histone deacetylase-containing complexes.
      Hakimi, Mohamed-Ali, et al.
      J. Biol. Chem., 278: 7234-9 (2003)  2003

      Show Abstract
      12493763 12493763

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies