05-1925 | Anti-Lactoylglutathione lyase Antibody, clone Glo1a

05-1925
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1925
      DescriptionAnti-Lactoylglutathione lyase Antibody, clone Glo1a
      Alternate Names
      • Glx I
      • Ketone-aldehyde mutase
      • S-D-lactoylglutathione methylglyoxal lyase
      • glyoxalase I
      • glyoxalase domain containing 1
      • lactoyl glutathione lyase
      Background InformationLactoylglutathione lyase is a detoxifying enzyme involved in the glyoxalase system and is responsible for the catalysis of methylglyoxal and lactate. Lactoylglutathione lyase is also referred to as Glyoxalase I.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationDetect the Lactoylglutathione lyase protein using this Anti-Lactoylglutathione lyase, clone Glo1a validated for use in WB & ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (Case Western Reserve University)
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human Lactoylglutathione lyase.
      EpitopeUnknown
      CloneGlo1a
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Lactoylglutathione lyase.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq].
      Gene Symbol
      • GLO1
      • Aldoketomutase
      • GLOD1
      • GLYI
      • Methylglyoxalase
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.

      CATALYTIC ACTIVITY: (R)-S-lactoylglutathione = glutathione + methylglyoxal.

      COFACTOR: Binds 1 zinc ion per subunit.

      PATHWAY: Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.

      SUBUNIT STRUCTURE: Homodimer.

      POLYMORPHISM: Exists in three separable isoforms which originate from two alleles in the genome. These correspond to two homodimers and one heterodimer composed of two subunits showing different electrophoretic properties.

      SEQUENCE SIMILARITIES: Belongs to the glyoxalase I family.
      Molecular Weight~ 21 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.1 µg/ml of this antibody detected Lactoylglutathione lyase on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications