06-333 Anti-MAP Kinase 2/Erk2 Antibody

200 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WBRbPurifiedPolyclonal Antibody
      Catalogue Number06-333
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-MAP Kinase 2/Erk2 Antibody
      Product Information
      Presentation0.02M phosphate buffer, pH 7.6, 0.25M NaCl, 0.1% sodium azide
      ApplicationAnti-MAP Kinase 2/Erk2 Antibody detects level of MAP Kinase 2/Erk2 & has been published & validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenRecombinant mouse ERK2
      SpecificityMAP Kinase encoded by the mapk gene
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
      Gene Symbol
      • MAPK1
      • p38
      • ERK
      • P42MAPK
      • ERK2
      • p40
      • PRKM1
      • p41mapk
      • PRKM2
      • MAPK2
      • p42-MAPK
      • p41
      • ERK-2
      • ERT1
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
      COFACTOR: Magnesium (By similarity).
      SIZE: 360 amino acids; 41390 Da
      SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
      DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
      PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight42kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on mouse 3T3/A31 fibroblasts, human A431 carcinoma cells or rat L6 skeletal fibroblasts
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-MAP Kinase 2/Erk2 (rabbit polyclonal IgG) - 24373512437351
      Anti-MAP Kinase 2/Erk2 (rabbit polyclonal IgG)2862336
      Anti-MAP Kinase 2/Erk2 (rabbit polyclonal IgG)2879677
      Anti-MAP Kinase 2/Erk2 - 1456214562
      Anti-MAP Kinase 2/Erk2 - 1592215922
      Anti-MAP Kinase 2/Erk2 - 2359923599
      Anti-MAP Kinase 2/Erk2 - 25240692524069
      Anti-MAP Kinase 2/Erk2 - 30634163063416
      Anti-MAP Kinase 2/Erk2 -26135692613569
      Anti-MAP Kinase 2/Erk2 -26765872676587


      Reference overviewApplicationPub Med ID
      Tyrosine phosphorylation profiling via in situ proximity ligation assay.
      Elfineh, L; Classon, C; Asplund, A; Pettersson, U; Kamali-Moghaddam, M; Lind, SB
      BMC cancer  14  435  2014

      Show Abstract
      24928687 24928687
      Granulosa cell expression of G1/S phase cyclins and cyclin-dependent kinases in PMSG-induced follicle growth.
      Jennifer D Cannon,Mary Cherian-Shaw,Tara Lovekamp-Swan,Charles L Chaffin
      Molecular and cellular endocrinology  264  2007

      Show Abstract
      17084963 17084963
      Synergistic inhibition of human melanoma proliferation by combination treatment with B-Raf inhibitor BAY43-9006 and mTOR inhibitor Rapamycin.
      Molhoek, KR; Brautigan, DL; Slingluff, CL
      Journal of translational medicine  3  39  2005

      Show Abstract Full Text Article
      16255777 16255777
      The beta3-adrenergic receptor activates mitogen-activated protein kinase in adipocytes through a Gi-dependent mechanism
      Soeder, K. J., et al
      J Biol Chem, 274:12017-22 (1999)  1999

      Immunoblotting (Western)10207024 10207024
      Distinct mechanisms of activation of Stat1 and Stat3 by platelet-derived growth factor receptor in a cell-free system.
      Vignais, M L and Gilman, M
      Mol. Cell. Biol., 19: 3727-35 (1999)  1999

      Show Abstract
      Immunoprecipitation10207096 10207096
      The Syk protein tyrosine kinase is essential for Fcgamma receptor signaling in macrophages and neutrophils
      Kiefer, F., et al
      Mol Cell Biol, 18:4209-20 (1998)  1998

      Immunoblotting (Western)9632805 9632805
      Tumor necrosis factor signaling to stress-activated protein kinase (SAPK)/Jun NH2-terminal kinase (JNK) and p38. Germinal center kinase couples TRAF2 to mitogen-activated protein kinase/ERK kinase kinase 1 and SAPK while receptor interacting protein associates with a mitogen-activated protein kinase kinase kinase upstream of MKK6 and p38.
      Yuasa, T, et al.
      J. Biol. Chem., 273: 22681-92 (1998)  1998

      Show Abstract
      Immunoblotting (Western)9712898 9712898
      Suppression of c-Myc-induced apoptosis by Ras signalling through PI(3)K and PKB
      Kauffmann-Zeh, A., et al
      Nature, 385:544-8 (1997)  1997

      Immunoblotting (Western)9020362 9020362
      An incomplete program of cellular tyrosine phosphorylations induced by kinase-defective epidermal growth factor receptors
      Wright, J. D., et al
      J Biol Chem, 270:12085-93 (1995)  1995

      IP-Kinase Assay7538132 7538132
      The serine/threonine phosphatase inhibitor, calyculin A, inhibits and dissociates macrophage responses to lipopolysaccharide.
      Barber, S A, et al.
      J. Immunol., 155: 1404-10 (1995)  1995

      Show Abstract
      Immunoblotting (Western)7636205 7636205

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      Product Families


      Life Science Research > Antibodies and Assays > Primary Antibodies