05-675 | Anti-MBP Antibody, clone SKB3

05-675
50 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-675
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-MBP Antibody, clone SKB3
      References
      Product Information
      FormatPurified
      Control
      • Brain tissue
      PresentationProtein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.02M Phosphate Buffer, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA containing no preservatives.
      Applications
      ApplicationDetect MBP using this Anti-MBP Antibody, clone SKB3 validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPeptide corresponding to the human myelin basic protein sequence containing amino acid Thr98
      CloneSKB3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes MBP.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
      Gene Symbol
      • MBP
      • MGC99675
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02686 # The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, each of them having maybe a specialized function. Induces T-cell proliferation.
      SIZE: 304 amino acids; 33117 Da
      SUBUNIT: Homodimer; isoform 3 exists as a homodimer.
      SUBCELLULAR LOCATION: Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.
      TISSUE SPECIFICITY: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.DEVELOPMENTAL STAGE: Expression turns on abruptly in fetus of 14 to 16 weeks. Even smaller isoforms seem to be produced during embryogenesis, some of these persisting in the adult. Expression of isoform MBP2 is more evident at 16 weeks and its relative proportion declined thereafter.
      PTM: Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases in aging, making the protein more cationic. & The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6). & Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
      DISEASE: SwissProt: P02686 # The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
      SIMILARITY: SwissProt: P02686 ## Belongs to the myelin basic protein family.
      Molecular Weight18-20 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from mouse and rat brain tissue preparations
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-MBP, clone SKB3 2470906
      Anti-MBP, clone SKB3 (mouse monoclonal IgG1#954;) - DAM1828795 DAM1828795
      Anti-MBP, clone SKB3 (mouse monoclonal IgG1k) 2919010
      Anti-MBP, clone SKB3 (mouse monoclonal IgG1k) - 2012464 2012464
      Anti-MBP, clone SKB3 (mouse monoclonal IgG1k) - 2106957 2106957
      Anti-MBP, clone SKB3 (mouse monoclonal IgG1k) - 2198985 2198985
      Anti-MBP, clone SKB3 (mouse monoclonal IgG1k) - 2361493 2361493
      Anti-MBP, clone SKB3 - 2054512 2054512
      Anti-MBP, clone SKB3 - 22368 22368
      Anti-MBP, clone SKB3 - DAM1661041 DAM1661041

      References

      Reference overviewPub Med ID
      Targeting endothelial junctional adhesion molecule-A/ EPAC/ Rap-1 axis as a novel strategy to increase stem cell engraftment in dystrophic muscles.
      Giannotta, Monica, et al.
      EMBO Mol Med, 6: 239-58 (2014)  2014

      Show Abstract
      24378569 24378569
      Human neural crest stem cells derived from human ESCs and induced pluripotent stem cells: induction, maintenance, and differentiation into functional schwann cells.
      Liu, Q; Spusta, SC; Mi, R; Lassiter, RN; Stark, MR; Höke, A; Rao, MS; Zeng, X
      Stem cells translational medicine  1  266-78  2012

      Show Abstract
      23197806 23197806
      Alcohol exposure decreases CREB binding protein expression and histone acetylation in the developing cerebellum.
      Guo, W; Crossey, EL; Zhang, L; Zucca, S; George, OL; Valenzuela, CF; Zhao, X
      PloS one  6  e19351  2011

      Show Abstract
      21655322 21655322
      Activation of multiple protein kinases during the burst in protein phosphorylation that precedes the first meiotic cell division in Xenopus oocytes.
      Cicirelli, M F, et al.
      J. Biol. Chem., 263: 2009-19 (1988)  1988

      2448302 2448302
      Cyclic AMP decreases the phosphorylation state of myelin basic proteins in rat brain cell cultures.
      Ulmer, J B, et al.
      J. Biol. Chem., 262: 1748-55 (1987)  1987

      Show Abstract
      2433287 2433287
      Identification of multiple in vivo phosphorylation sites in rabbit myelin basic protein.
      Martenson, R E, et al.
      J. Biol. Chem., 258: 930-7 (1983)  1983

      Show Abstract
      6185481 6185481

      Technical Info

      Title
      Derivation of oligodendrocyte progenitor cells from a human neural stem cell line

      Newsletters / Publications

      Title
      Cellutions - The newsletter for Cell Biology Researchers Volume 3: 2011

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies